Author/Editor		Hajdinjak, Tine; Randl, Tomaž
Title		Histokemična identifikacija motoričnih snopičev perifernih živcev
Translated title		Histochemical identification of motor fascicles in peripheral nerves
Type		članek
Source		Med Razgl
Vol. and No.		Letnik 36, št. 1
Publication year		1997
Volume		str. 15-35
Language		slo
Abstract		Distinguishing between motor and sensory fascicles is essential to successful peripheral nerve reconstruction. Acetylthiocholine staining methods based on the Karnovsky procedure have been increasingly used for the purpose. Our aim was to reduce the staining time, which is at least 45 minutes, so that the method could be used intraoperatively. We studied influence of staining time, temperature and components of staining solution on the results of staining. An additional oxidation step was introduced. The new method was evaluated by blind examinations of 5 differently stained groups of 30 randomized specimens of mixed and sensory rat nerves. Optimum parameters of the staining process were defined. The new modification with the added oxidation step had the same sensitivity and specificity rates as the more time-consuming standard methods and yielded sharper staining in only 30 minutes. The added oxidation step significantly reduces the time of motor and sensory fibre differentiation, and is suitable for clinical use.
Summary		Razlikovanje med motoričnimi in senzibilnimi živčnimi snopiči je pomembno za doseganje čim boljših rezultatov zdravljenja prekinjenih perifernih živcev. uveljavlja se razlikovanje s histokemičnimi metodami, zlasti na osnovi barvanja po Karnovskem. Te metode trajajo vsaj 45 minut. Naš namen je bil skrajšati čas barvanja, da bi omogočili uporabo metode med operacijo. Proučili smo vpliv časa barvanja, temperature in sestavin inkubacijske raztopine na rezultate barvanja. Uvedli smo dodatno oksidacijsko stopnjo. Novo spremenjeno metodo smo ovrednotili s slepim pregledom 5 različno obarvanih skupin s po 30 naključno razporejenimi vzorci mešanih in senzibilnih živcev podgan. Določili smo parametre za najprimernejši postopek barvanja. Nova spremenjena metoda z dodatno oksidacijsko stopnjo ima enako občutljivost in specifičnost kot dolgotrajnejše metode in prikaže ostrejše obarvanje v samo 30 minutah. Dodatna oksidacijska stopnja pomembno skrajša čas histokemičnega barvanja za razlikovanje med motoričnimi in senzibilnimi snopiči in je primerna za klinično uporabo.
Descriptors		SCIATIC NERVE MOTOR NEURONS HISTOCYTOCHEMISTRY RATS, WISTAR ACETYLTHIOCHOLINE STAINING TETRAISOPROPYLPYROPHOSPHAMIDE TISSUE FIXATION