Author/Editor		Ravnik, Mateja
Title		Dokazovanje klamidijske DNA iz rodil pri ženski
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta in Biotehniška fakulteta
Publication year		1997
Volume		str. 83
Language		slo
Abstract		Chlamydia trachomatis is one of the most common causes of sexually transmitted disease. Cell culture assay has been considered to be the standard method for the detection of C. trachomatis. The use of the in vitro DNA amplification methods has shown higher sensitivity than the cell culture assay. In this study commercially available polymerase chain reaction Amplicor assay, for the detection of C. trachomatis plasmid DN, was compared to the in-house polymerase chain reaction assay for the detection of C. trachomatis chromosome DNA and also to the classic cell culture assay and to the direct immunofluorescence. Two groups of asymptomatic women were included in this study: young women under 20 years of age at high risk for C. trachomatis infection and the infertile women. In these women C. trachomatis infection of the lower genital tract could progress to the upper tract, where C. trachomatis infection could be related to the tubal factor infertility. This study has shown that commercially available polymerase chain reaction Amplicor assay was more sensitive than the cell culture and the direct immunofluorescence. The diagnostic sensitivity of Amplicor asasy, direct immunofluorescence and cell culture with cervical and urethral specimens was 100%, 77% and 62%, respectively. The sensitivity of Amplicor assay and direct immunofluorescence with urine specimens was 100% and 33%. The study has elucidated that Amplicor assay was the most suitable diagnostic method; urine has been shown to be the most suitable specimen. The results were in good correlation with the findings obtained with cervical and urethral specimens. Three different amplification strategies were used for the detection of chlamydial DNA. (Abstract truncated at 2000 characters).
Descriptors		INFERTILITY, FEMALE CHLAMYDIA INFECTIONS CHLAMYDIA TRACHOMATIS DNA ADULT ADOLESCENCE POLYMERASE CHAIN REACTION PLASMIDS CHROMOSOMES, HUMAN FLUORESCENT ANTIBODY TECHNIQUE VAGINAL SMEARS URINE