Author/Editor		Krejci, Eric; Thomine, Sebastien; Boschetti, Nicola; Legay, Claire; Sketelj, Janez; Massoulie, Jean
Title		The mammalian gene of acetylcholinesterase-associated collagen
Type		članek
Source		J Biol Chem
Vol. and No.		Letnik 272, št. 36
Publication year		1997
Volume		str. 22840-7
Language		eng
Abstract		The collagen-tailed or asymmetric forms (A) represent a major component of acetylcholinesterase (AChE) in the neuromuscular junction of higher vertebrates. They are hetero-oligomeric molecules, in which tetramers of catalytic subunits of type T (AChE/T) are attached to the subunits of a tripe-stranded collagen "tail". We report the cloning of a rat AChE-associated collagen subunit, Q. We show that collagen tails are encoded by a single gene, COLQ. The ColQ subunits form homotrimers and readily form collagen-tailed AChE, when coexpressed with rat AChE/T. We found that the same ColQ subunits are incorporated, in vivo, in asymmetric forms of both AChE and butytylcholinesterase. A splice variant from the COLQ gene encodes a proline-rich AChE attachment domain without the collagen domain but doues not represent the membrane anchor of the brain tetramer. The COLQ gene is expressed in cholinergic tissues, brain, muscle, and heart, and also in noncholinergic tissues such as lung and testis.
Descriptors		ACETYLCHOLINESTERASE COLLAGEN RATS, WISTAR BASE SEQUENCE DNA PROBES AMINO ACID SEQUENCE DNA, COMPLEMENTARY MOLECULAR SEQUENCE DATA RNA SPLICING SEQUENCE HOMOLOGY, AMINO ACID TORPEDO