Author/Editor		Seme, Katja; Poljak, Mario; Čelan-Lucu, Branka; Lešničar, Gorazd; Brinovec, Vladimir; Matičič, Mojca; Meglič-Volkar, Jelka; Žužek-Rešek, Saša; Štepec, Srečko; Baklan, Zvonko; Andoljšek, Dušan; Benedik-Dolničar, Majda; Dovč, Peter; Koren, Srečko
Title		Genomska tipizacija izolatov virusa hepatitisa C v Sloveniji
Translated title		Genotyping of hepatitis C virus isolates in Slovenia
Type		članek
Source		Med Razgl
Vol. and No.		Letnik 36, št. 3
Publication year		1997
Volume		str. 345-62
Language		slo
Abstract		Genotyping of hepatitis C virus (HCV) isolates is of particular interest for epidemiology. Because of extreme genome variability of HCV genome, each genotyping method should be evaluated in local setting before being introduced as a routine diagnostic procedure or a tool for large-scale epidemiological studies. This comparative study of four most widely used HCV genotyping assays, employed on 40 HCV isolates in Slovenia, showed that none of them was realy suitable for genotyping Slovenian HCV isolates. Thanks to its satisfactory sensitivity, specificity and simplicity, the line probe assay was found to be the most appropriate tool for both routine and large-scale epidemiological studies. Using this assay the prevalence of HCV genotypes in Slovenia was studied on 226 individuals infected with HCV Significant differences in HCV genotype distribution observed between epidemiological groups in Slovenia indicate a close relationship between individual HCV genotypes in Slovenia was studied on 226 individuals infected with HCV. Significant differences in HCV genotype distribution observed between epidemiological groups in Slovenia indicate a close relationship between individual HCV gentoypes and certain routes of viral transmission.
Summary		Opredelitev genotipa virusa hepatitisa C (HCV) je pomembna v raziskovanju epidemiologije okužbe s tem virusom oz. za natančno spremljanje ter razjasnitev načina prenosa in širjenja okužbe. Zaradi spremenljivosti genoma in neenakomerne zemljepisne porazdelitve genotipov HCV je treba pred začetkom kakrčnekoli epidemiološke raziskave v vsaki državi preveriti učinkovitost različnih tipizacijskih metod s pilotsko raziskavo in ob tem ugotoviti tudi okvirno prisotost in delež posameznih genotipov HCV S primerjalno raziskavo, ki smo jo izvedli na 40 izolatih RNA HCV iz Slovenije, smo primerjali učinkovitost štirih posrednih metod genomske tipizacije in ugotovili, da s posameznimi metodami ni mogoče dokončno opredeliti vseh genotipov oz. podtipov HCV, prisotnnih v Sloveniji. Izkazalo se je, da je v našem prostoru najbolj primerna metoda genomske tipizacije reverzni dot-blot s tipsko specifičnimi DNA-lovkami za 5'nekodirajoči del genoma HCV. Z izbrano strategijo genomske tipizacije smo nato določili genotip/podtip HCV pri 226 slovenskih bolnikih, ki so se okužili s HCV na različne načine oz. so pripadali različnim epidemiološkim skupinam. Razporeditev genotipov HCV se v SLoveniji razlikuje med posameznimi epidemiološkimi skupinami bolnikov. Rezultati raziskave kažejo na povezavo med posameznimi genotipi HCV in določenimi načini prenosa virusa.
Descriptors		HEPATITIS C-LIKE VIRUSES GENOTYPE SLOVENIA HEPATITIS C HEMODIALYSIS HEMOPHILIA SUBSTANCE ABUSE, INTRAVENOUS