Author/Editor		Romih, Rok
Title		Endovakuolarne poti v površinskih urotelijskih celicah miši
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1993
Volume		str. 48
Language		slo
Abstract		In superficial cells of the mouse urinary bladder the origin of late endosomes - multivesicular bodies and turn-over of luminal plasmalemma are not yet clear. Undoubtedly old luminal plasmalemma is removed via multivesicular bodies which are part of endovacuolar apparatus. Therefore in these cells endovacuolar compartments, their interconnections and their relations to multivesicular bodies were studied. The effect of cell differentiation on intensiveness of endovacuolar processes was analyzed. Different morphological and cytochemical electron microscopical techniques were used. Six groups of urothelia were analyzed corresponding to days 0 (day of partus), 1, 2, 3, 5 and > 21 (adult animals). We were able to demonstrate that endocytic pathways of fluid and of membranes are the same. Luminal plasmalemma and membranes of endosomes are similar in respect to the composition of glucoconjugates. Only minor part of luminal plasmalemma is included in endocytosis, which is relatively slow. During endocytosis asymmetrical unit membranes and symmetrical unit membranes are internalized exclusively in uncoated vesicles. From asymmetrical unit membranes and symmetrical unit membranes cupshaped early endosomes are created. Early endosomes and late endosomes - multivesicular bodies communicate through polymorphic cell compartmets. In the formation of multivesicular bodies membranes of early endosomes are taking part. Number and size of multivesicular bodies are influenced by the degree of differentiation in superficial urothelial cells.
Descriptors		BLADDER ENDOSOMES CELL DIFFERENTIATION CELL MEMBRANE ENDOCYTOSIS MICE MICROSCOPY, ELECTRON FREEZE FRACTURING PEROXIDASE GOLD COLLOID ACID PHOSPHATASE HISTOCYTOCHEMISTRY