Author/Editor | Romih, Rok | |
Title | Obnova in diferenciacija urotelijskih celic po učinkovanju natrijeve soli saharina in ciklofosfamida | |
Type | monografija | |
Place | Ljubljana | |
Publisher | Medicinska fakulteta | |
Publication year | 1996 | |
Volume | str. 122 | |
Language | slo | |
Abstract | Normal urinary bladder urothelium of adult mammals is stable and differentiated epithelium. Experimentally induced lesions of urothelium lead to quick regeneration but cell processes that are involved are poorly understood. Thus we studied the mechanisms of action of sodium saccharin (NaSac) and cyclophosphamide (CP) and cellular events that bring about the regeneration and the recurrent differentiation of normal urothelium. Urothelial lesions were induced in male rats by high dietary NaSac level or by one intraperitoneal injection of CP. Because urine plays a critical role in urothelial response to the treatment its biochemical and spectroscopic analyses were done. Urothelial changes were studied by ligh and electron microscopy using morphological and immunocytochemical methods. Our results show that NaSac changes the composition of the urine and enables the formation of different precipitates which in turn induce focal exfoliation of superficial cells from ventral parts of urinary bladders. In contrast CP induces diffuse exfoliation of all urothelial layers. In both cases, however, undifferentiated cells with untickened apical plasma membrane become directly exposed to the urine. Regardless the treatment procedure regeneration and differentiation take place at the damaged areas. The proliferation of basal and intermediate cells is increased which leads to mild urothelial hyperplasia. Proliferation is accompanied by differentiation that depends on apical plasma membrane specificity, reorganisation of cell compartments, altered membrane dynamics and synthesis of different cytokeratins. This is reflected in successive changes of different superficial cell types. (Abstract truncated at 2000 characters.) | |
Descriptors | BLADDER CYCLOPHOSPHAMIDE SACCHARIN CELL DIFFERENTIATION PROLIFERATING CELL NUCLEAR ANTIGEN RATS, WISTAR CELL MEMBRANE HYPERPLASIA CYTOSKELETON ENDOCYTOSIS KERATIN MICROSCOPY MICROSCOPY, ELECTRON IMMUNOHISTOCHEMISTRY URINE ENDOSOMES FREEZE FRACTURING |