Author/Editor     Romih, Rok
Title     Obnova in diferenciacija urotelijskih celic po učinkovanju natrijeve soli saharina in ciklofosfamida
Type     monografija
Place     Ljubljana
Publisher     Medicinska fakulteta
Publication year     1996
Volume     str. 122
Language     slo
Abstract     Normal urinary bladder urothelium of adult mammals is stable and differentiated epithelium. Experimentally induced lesions of urothelium lead to quick regeneration but cell processes that are involved are poorly understood. Thus we studied the mechanisms of action of sodium saccharin (NaSac) and cyclophosphamide (CP) and cellular events that bring about the regeneration and the recurrent differentiation of normal urothelium. Urothelial lesions were induced in male rats by high dietary NaSac level or by one intraperitoneal injection of CP. Because urine plays a critical role in urothelial response to the treatment its biochemical and spectroscopic analyses were done. Urothelial changes were studied by ligh and electron microscopy using morphological and immunocytochemical methods. Our results show that NaSac changes the composition of the urine and enables the formation of different precipitates which in turn induce focal exfoliation of superficial cells from ventral parts of urinary bladders. In contrast CP induces diffuse exfoliation of all urothelial layers. In both cases, however, undifferentiated cells with untickened apical plasma membrane become directly exposed to the urine. Regardless the treatment procedure regeneration and differentiation take place at the damaged areas. The proliferation of basal and intermediate cells is increased which leads to mild urothelial hyperplasia. Proliferation is accompanied by differentiation that depends on apical plasma membrane specificity, reorganisation of cell compartments, altered membrane dynamics and synthesis of different cytokeratins. This is reflected in successive changes of different superficial cell types. (Abstract truncated at 2000 characters.)
Descriptors     BLADDER
CYCLOPHOSPHAMIDE
SACCHARIN
CELL DIFFERENTIATION
PROLIFERATING CELL NUCLEAR ANTIGEN
RATS, WISTAR
CELL MEMBRANE
HYPERPLASIA
CYTOSKELETON
ENDOCYTOSIS
KERATIN
MICROSCOPY
MICROSCOPY, ELECTRON
IMMUNOHISTOCHEMISTRY
URINE
ENDOSOMES
FREEZE FRACTURING