Author/Editor		Vitas, Marko; Pajič, Tadej; Kelly, Steven L; Komel, Radovan
Title		11beta-hydroxysteroid dehydrogenase activity in progesterone biotransformation by the filamentous fungus Cochliobolus lunatus
Type		članek
Source		J Steroid Biochem Mol Biol
Vol. and No.		Letnik 63, št. 4-6
Publication year		1997
Volume		str. 345-50
Language		eng
Abstract		Progesterone biotransformation was examined in relation to hydroxylating and dehydrogenating enzymes of Cochliobolus lunatus. 11beta-hydroxysteroid dehydrogenase activity (11beta-HSD) was located in cytosolic fraction and was NADP-dependent, inducible by progesterone and apparently unidirectional. Several inhibitors of 11beta-hydroxysteroid dehydrogenase were tested; furosemide, glycyrrhizic-acid and carbenoxolone did not influence the dehydrogenation of 11beta-hydroxy-4-pregnene-3,20-dione to 4-pregnene-3,11,20-trione, although grapefruit juice significantly reduced the rate of progesterone hydroxylation.
Descriptors		ASCOMYCETES BIOTRANSFORMATION PROGESTERONE HYDROXYSTEROID DEHYDROGENASES DENSITOMETRY SPECTRUM ANALYSIS, MASS MICROSOMES CYTOSOL