Author/Editor     Vitas, Marko; Pajič, Tadej; Kelly, Steven L; Komel, Radovan
Title     11beta-hydroxysteroid dehydrogenase activity in progesterone biotransformation by the filamentous fungus Cochliobolus lunatus
Type     članek
Source     J Steroid Biochem Mol Biol
Vol. and No.     Letnik 63, št. 4-6
Publication year     1997
Volume     str. 345-50
ISSN     0960-0760
Language     eng
Abstract     Progesterone biotransformation was examined in relation to hydroxylating and dehydrogenating enzymes of Cochliobolus lunatus. 11beta-hydroxysteroid dehydrogenase activity (11beta-HSD) was located in cytosolic fraction and was NADP-dependent, inducible by progesterone and apparently unidirectional. Several inhibitors of 11beta-hydroxysteroid dehydrogenase were tested; furosemide, glycyrrhizic-acid and carbenoxolone did not influence the dehydrogenation of 11beta-hydroxy-4-pregnene-3,20-dione to 4-pregnene-3,11,20-trione, although grapefruit juice significantly reduced the rate of progesterone hydroxylation.
Descriptors     ASCOMYCETES
BIOTRANSFORMATION
PROGESTERONE
HYDROXYSTEROID DEHYDROGENASES
DENSITOMETRY
SPECTRUM ANALYSIS, MASS
MICROSOMES
CYTOSOL