Author/Editor     Gubina, Marija; Urbančič-Rovan, Vilma; Tomič, Viktorija
Title     Anaerobes isolated from clinical material over three years
Type     članek
Source     Anaerobe
Vol. and No.     Letnik 3
Publication year     1997
Volume     str. 79-81
Language     eng
Abstract     Data on anaerobic bacteria isolated from clinical specimens at the bacteriology department within the 3-year period (1992-1994) were analysed. Anaerobic cultivation was carried out in all aspirates and swabs were transferred in transport media in syringes or blood cultures. Established growth occurred in all samples cultivated in thioglycollate broth after 4 days of incubation. Cultivation methods included enrichment media, GasPak jar, and API (BioMerieux) for final identification. A sulfite-reduction test using the Wilson-Blair medium and the Ellner-Smith sporulationmedium was also used for the isolation of Clostridium perfrigens. Anaerobes were diagnosed in 899 samples. Wound swabs (266 samples) and aspirates (106 samples) were the most common clinical material used. In total, 964 anaerobes were isolated: Peptostreptococus species (299 strains), Eubacterium species (188 strains), Propionibacterium species (153 strains), Bacteriodes fragilis (149 strains), Bacteriodes species (95 strains) and Clostridium perfringes (80 strains).
Descriptors     BACTERIA, ANAEROBIC
CULTURE MEDIA
CLOSTRIDIUM PERFRINGENS
BACTEROIDES
PROPIONIBACTERIUM
PEPTOSTREPTOCOCCUS
EUBACTERIUM