Author/Editor		Rupnik, Maja; Braun, Veit; Soehn, Florian; Janc, Miha; Hofstetter, Monika; Laufenberg-Feldmann, Rita; von Eichel-Streiber, Christoph
Title		Characterization of polymorphism in the toxin A and B genes of Clostridium difficile
Type		članek
Source		Fems Microbiol Lett
Vol. and No.		Letnik 148
Publication year		1997
Volume		str. 197-202
Language		eng
Abstract		We have used six independent polymerase chain reactions (A1-A3 and B1-B3) for amplificantion of the entire sequence of the two toxin genes tcdA and tcdB of several Clostridium difficile strains. With this approach we have detected (1) restriction site polymorphisms which are distributed all over the genes, and (2) deletions that could be found only in tcdA. Characteristic differences between strains were mainly focused to the 5' third of tcdB (B1 fragment) and/or the 3' third of tcdA (A3 fragment). The possible use of our approach for typing of C. difficile toxin genes is discussed.
Descriptors		CLOSTRIDIUM DIFFICILE BACTERIAL TOXINS ENTEROTOXINS GENES, BACTERIAL POLYMERASE CHAIN REACTION POLYMORPHISM (GENETICS) POLYMORPHISM, RESTRICTION FRAGMENT LENGTH