Author/Editor		Jaki, Polona
Title		Vpliv radiojodne terapije na vrsto protiteles proti receptorju za tirotropin pri bolnikih z bazedovko
Translated title		The effect of radioiodine (131J) therapy upon thyroid-stimulating hormone receptor (TSH-R) related antibody (TRAb) was studied in forty-eight patients with Graves' disease
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1997
Volume		str. 76
Language		slo
Abstract		The effect of radioiodine (131J) therapy upon thyroid-stimulating hormone receptor (TSH-R) related antibody (TRAb) was studied in forty-eight patients with Graves' disease. TRAb was measured before radioiodine therapy and six months thereafter; follow-up of patients was conducted one year after the therapy. A radioreceptor assay was used to detect TRAb in sera, and a biological assay to detect biological activity of TRAb, for which we isolated IgG from sera by double separation on DEAE-Sephadex and precipitation by ammonium sulphate. IgG were tested on Chinese Hamster Ovary cells transfected with the clone of human TSH-R (CHO-R). With the radioreceptor assay, the antibodies which were capable of binding to TSH-R, were determined and defined as TRAb. With the biological assay, we categorised the antibodies according to their effect on cAMP synthesis. Antibodies which caused an increase in the concentration of cAMP were defined as stimulating antibodies (TSAb). For those that blocked the TSH-induced increase in the concentration of cAMP we determined an index of inhibition, and defined them as blocking antibodies (TSBAb). The majority of our patients had elevated levels of TRAb, TSAb and TSBAb before the radioiodine therapy. A statistical analysis revealed no significant difference (p>0,05) in the level of antibodies between these two groups of patients prior to the therapy. After the therapy some patients remained hyperthyroid, whereas others became hypothyroid. The pattern of changes in TSAb activity was similar in both groups of patients after the therapy: increased acitivity, decreased activity, or similar level of activity compared to the activity prior to the therapy. The TSBAb were positive in 40% of hyperthyroid patients, and only in 9,5% of hypothyroid patients after radioiodine therapy. Aii patients with positive the TSBAb aiso had increased stimuiating activity of TSAb. (Abstracts truncated at 2000 characters)
Descriptors		GRAVES' DISEASE IODINE RADIOISOTOPES RECEPTORS, THYROTROPIN ADULT IGG FOLLOW-UP STUDIES RADIOLIGAND ASSAY CYCLIC AMP HYPERTHYROIDISM HYPOTHYROIDISM