Author/Editor		Kveder, Marina; Pifat, Greta; Pečar, Slavko; Schara, Milan; Ramos, Pilar; Esterbauer, Hermann
Title		The interaction of lower alcohols vith apoB in spin labeled human plasma low density lipoproteins (LDL)
Type		članek
Source		Chem Phys Lipids
Vol. and No.		Letnik 87
Publication year		1997
Volume		str. 125-35
Language		eng
Abstract		In this study the interaction of alcohol with the macromolecular lipid-protein assembly represented by human plasma low density lipoproteins (LDL) was investigated. The spin label which covalently binds to the side chain amino group of lysines as well as terminal amino groups was attached to the spin labeled apoprotein (apoB) of native LDL in order to observe the protein component in the electron spin resonance (ESR) spectrum. The interaction of different lower alcohols (methanol, ethanol, propanol and butanol) with the spin labeled LDL was studied for two alcohol concentrations (0.3 and 3.0M). The ESR spectra indicate a decrease of the hyperfine splitting and narrowing of the linewidth upon the action of alcohol that leads to the conclusion that alcohol provokes a change in the apoB conformation. These findings are explained by following the arguments of the phospolipid mediated mechanism of alcohol action, through the modulation of the lipid packing free volume which results in the protein conformational change.
Descriptors		APOLIPOPROTEINS ALCOHOLS LIPOPROTEINS, LDL SPIN LABELS CHOLESTEROL ESTERS FATTY ACIDS MOLECULAR STRUCTURE NITROBENZENES OXALATES PHOSPHOLIPIDS PROTEIN CONFORMATION TRIGLYCERIDES TRYPSIN NICKEL ELECTRON SPIN RESONANCE SPECTROSCOPY