Author/Editor		Milisav, I; Jones, MH; Affara, NA
Title		Characterization of a novel human dynein-related gene that is specifically expressed in testis
Type		članek
Source		Mamm Genome
Vol. and No.		Letnik 7
Publication year		1996
Volume		str. 667-72
Language		eng
Abstract		A novel dynein-related transcript (designated DNELI) from human adult testis has been identified that can encode a protein with a size of 91087 Da. The complete nucleotide sequence of the open reading frame is the first to be described for a human dynein-related gene. Northern blot analysis of mRNA from 16 different tissues has shown that DNELLI is expressed specifically in testis.Analysis of somatic cell hybrids has mapped DNELI to Chromosome (Chr) 17. Analysis of a panel of 129 whole genome radiation hybrid clones including 17q22-q25.3 has placed DNELI in 17q distal to the ERBA2l locus. DNELI shares a high degree of sequence identity and amino acid similarity with the C-terminal region of the outer arm axonemal dynein beta-heavy chains derived from sea urchin and other species, but not to any gene encoding dynein intermediate or light chains described to date. The close similarity of DNELI to the C-terminal part of the axonemal beta-heavy chain may suggest an origin from a common progenitor gene and the testis-specific pattern of expression a possible role in sperm development or motility.
Descriptors		DYNEIN ATPASE CHROMOSOMES, HUMAN, PAIR 17 TESTIS TRANSCRIPTION, GENETIC PROTEINS EVOLUTION DNA PRIMERS GENE EXPRESSION REGULATION SPERM MOTILITY SPERMATOGENESIS CHROMOSOME MAPPING BASE SEQUENCE AMINO ACID SEQUENCE MOLECULAR SEQUENCE DATA ORGAN SPECIFICITY SEA URCHINS SEQUENCE HOMOLOGY, AMINO ACID SEQUENCE HOMOLOGY, NUCLEIC ACID HYBRID CELLS