Author/Editor		Žohar-Čretnik, Tjaša; Štorman, Alenka
Title		Naš pristop k serološki diagnostiki okužbe s Toxoplasma gondii
Translated title		Our approach to serodiagnosis of the Toxoplasma gondii infection
Type		članek
Source		Med Razgl
Vol. and No.		Letnik 37, št. Suppl 1
Publication year		1998
Volume		str. 107-13
Language		slo
Abstract		Since December 15, 1995, serologic screening for the recent infection with Toxoplasma gondii has been regulated by law in Slovenia. The basic screening method for detection of specific IgM and IgG antibodies in the serum samples in the microbiology laboratory of the Institute of Public health in Celje is ELISA. According to results we decide on further testing. The specific IgA antibodies are detected by ELISA test, the titre of specific IgG antibodies is determined by indirect immunofluorescence test and an assay measuring avidity of specific IgG antibodies is used as needed. In the period from January 1, 1996 to November 1, 1997 we tested samples of 3914 pregnant women. Of them 1917 were found to be seropositive. We detected specific IgM antibodies in 54 cases but only 4 were classified as recent infections. Of 2003 seronegatives 33 (1.6%) were tested three times, 257 (12.8%) twice and 1719 only once during pregnancy. We believe that the reason for such results is irregular testing in seronegative women. As the interpretation of serologic test results is complex and difficult and the consequences of mistakes are severe, an agreement on appropriate methods should be reached in our country. An adequate board of consultants should also be our further aim.
Summary		Od 15. decembra 1995 je v državi uzakonjeno presejanje na svežo okužbo s Toxoplasma gondii. V mikrobiološkem laboratoriju Zavoda za zdravstveno varstvo v Celju presejalna metoda ELISA, s katero določamo prisotnost specifičnih protiteles IgM in IgG v serumu. Glede na rezultate se odločamo o dodatnem testiranju. Določamo titer specifičnih protiteles IgG s testom indirektne imunofluorescence, prisotnost specifičnih protiteles IgG. V obdobju od 1. januarja 1996 do 1. novembra 1997 smo preiskali serume 3914 nosečnic. Seropozitivnih je bilo 1917. Specifična protitelesa IgM smo zaznali pri 54 nosečnicah, a le pri štirih nismo mogli izključiti okužbe po zanostvi. Od 2003 seronegativnih je bilo pri nas trikrat testiranih 33 (1,6%), dvakrat 257 (12,8%) in le enkrat 1713 (85,6% nosečnic. Menimo, da zaradi preredkih kontrolnih pregledov pri seronegativnih nosečnicah nismo zaznali več svežih okužb. Ker je interpretacija rezultatov seroloških testov zahtevna, posledice napak pa hude, bi v državi potrebovali dogovor o enotnem in nadzorovanem načinu testiranja ter strokovno telo, kjer bi se lahko posvetovali o rezultatih, ki odstopajo od povprečja.
Descriptors		TOXOPLASMOSIS TOXOPLASMA SERODIAGNOSIS PREGNANCY ENZYME-LINKED IMMUNOSORBENT ASSAY IGG