| Author/Editor | Čebašek, Vita | |
| Title | Histokemična in morfometrična analiza mišice latissimus dorsi človeka | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 1997 | |
| Volume | str. 54 | |
| Language | slo | |
| Abstract | The human latissimus dorsi (LD) muscle is a large, fan-shaped muscle. Its fibres run in three main directions. Tgerefore it is commonly divided into superior, middle and lateral segment. During different phases of shoulder movement segments show different electromyographic (EMG) activity. Anatomical properties of LD muscle are already well known, because its muscle or musculocutaneous flaps are frequently used for reconstructions of soft-tissue defects. Less known, however, are its histochemical characteristics, which reflect functional properties of muscle. Additional information of LD muscle fibre type composition might be useful for functional reconstructions, i.e. experimental procedures, where contraction power of transferred LD muscle could be used. Autopsy samples of LD muscle from six males aged from 17 to 35 years and six from 43 to 60 years, who died suddendly, were analysed with the following methods: - histochemical reaction for myofibrillar adenosine triphosphatase (mATPase) in alkaline (pH9,4) and in acidic media (pH 4,5 and pH 4,3) to show fibre types 1, 2a, 2b and 2c, - histochemical reactions for NADH tetrazolium reductase (NADH-TR) and menadion linked alpha-glycerophosphate dehydrogenase (alpha-GPDH) to determine muscle metabolism, - immunoperoxidase staining of fibronectin for demonstrating capillaries. The percentage, diameter and cross-sectional area of fibre types, their arrangement inside the fascicle and relative activity of their metabolic enzymes were determined using a computer-adied morphometric and histophotometric methods. Estimation of capillary supply in realtion to fibre type also performed. In the younger group the percantage and cross-sectional area of fibre types were (1>2b>2a>2c).(Abstract truncated at 2000 characters.) | |
| Descriptors | MUSCLE, SKELETAL ADENOSINETRIPHOSPHATASE NADH TETRAZOLIUM REDUCTASE GLYCEROLPHOSPHATE DEHYDROGENASE FIBRONECTINS HISTOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY ADULT MIDDLE AGE MYOFIBRILS |