Author/Editor		Gruden, Kristina; Štrukelj, Borut; Ravnikar, Maja; Poljšak-Prijatelj, Mateja; Mavrič, Irena; Brzin, Jože; Pungerčar, Jože; Kregar, Igor
Title		Potato cysteine proteinase inhibitor gene family: molecular cloning, characterisation and immunocytochemical localisation studies
Type		članek
Source		Plant Mol Biol
Vol. and No.		Letnik 34
Publication year		1997
Volume		str. 317-23
Language		eng
Abstract		Potato cysteine proteinase inhibitors (PCPIs) represent a distinct group of proteins as they show no homology to any other known cysteine proteinase inhibitor superfamilies, but they all belong to the Kunitz-type soybean trypsin inhibitor family cDNA clones for five PCPIs have been isolated and sequenced. Amino acid substitutions occurring in the limited regions forming loops on the surface of these proteins suggest a further classification of PCPIs into three subgrouops. Accomulation of PCPI was observed in vacuoles of stems after treatment with jasmonic acid (JA) using immunocytochemical localisation, implying that these inhibitors are part of a potato defence mechanism against insects and pathogens. Genomic DNA analysis show that PCPIs form a multigene family and suggest that their genes do not possess any introns.
Descriptors		POTATOES MULTIGENE FAMILY CYSTEINE PROTEINASE INHIBITORS GENE EXPRESSION REGULATION, PLANT GENES, STRUCTURAL, PLANT AMINO ACID SEQUENCE CLONING, MOLECULAR GENOME, PLANT IMMUNOCHEMISTRY INTRACELLULAR FLUID MOLECULAR SEQUENCE DATA SEQUENCE ANALYSIS, DNA