Author/Editor     Benedičič, Desimira; Ravnikar, Maja; Gogala, Nada
Title     The regeneration of bean plants from meristem culture
Type     članek
Source     Phyton Horn
Vol. and No.     Letnik 37, št. 1
Publication year     1997
Volume     str. 151-60
Language     eng
Abstract     Benedičič D., Ravnikar M. and Gogala N. 1997. The regeneretion plants from meristem culture. - Ohyton (Horn, Austria) 37 (1): 151-160, 4 figures. - English with German summary. We studied the growth and development of Phaseolus vulgaris L. cv. Zorin meristems in vitro. Special attention was paid to the influence of different types of cytokinins and their concentration on bud induction, shoot growth and callus formation. The appropriate conditions of seed germination were established in order to separate seeds with various internal seed diseases prior to meristem isolation. Basal media was supplemented with 0.1-10 micromol/l benzyladenine, or with 1-10 micromol/l 6-(y, y-dimetylallyamino)-purine, or with combinations of benzyladenine and gibberellic acid or alpha-naphthalene acetic acid. Although the buds developed on all media used, the regeneration of plants able to survive in soil was achieved only when meristems were isolated on basal medium supplemented with 1 and 5 mocromol/l benzyladenine or 6-(y, y-dimethylallylaminol)-purine and with 20 micromol/l benzyladenine and 1.4 micromol/l gibberellic acid. The most vigorous plants developed when 1 micromol/l 6-(y, y-dimethylallylamino)-purine was used for initial meristem culture and was further incorporated in the elongation media. According to these results, we worked out a system for meristem regeneration, elongation and rooting, and prepared the protocol for production of mature bean plants.
Descriptors     LEGUMES
MERISTEM
TISSUE CULTURE
CYTOKININS
SEEDS
GERMINATION