Author/Editor		Kopač, Igor; Batista, Urška; Cvetko, Erika; Marion, Ljubo
Title		The effect of gingival retraction agents on fibroblast cell cultures
Type		članek
Source		In: EVDS. Proceedings of the 7th European congress of veterinary dentistry; 1998 May 15-16; Ljubljana. Ljubljana: Slovensko združenje veterinarjev za male živali,
Publication year		1998
Volume		str. 57-60
Language		eng
Abstract		To achieve an acceptable fixed restoration with a well adapted gingival margin, an accurate recording of the finishing line location and also a portion of the uncut tooth structure is required. A slight free gingival tissue displacement is desired to obtain a space for impression-making. The mechanicochemical method appears to be the most commonly used one. All the commonly used chemicals are highly acidic, with pH values from 0.8 to 3.2. The use of these agents represents a potential danger for cut dentine and periodontal tissues. The aim of the study was to compare the damaging effects of several most commonly used retractive agents and to assess the time of ibroblast cell destruction for the particular agent. Cell cultures of hamster lung fibroblast cells (V-79) were used. The following agents were tested: 25 % aluminium chloride (Racestyptine, Septodont France), 10 % aluminium chloride (Gingiva Liquid, Roeko, Germany), 20% aluminium sulphate, 8 % epinephrine (Surgident, USA), and sympathomimetic vasoconstrictor - 0,1 % tetrahydrozoline (Tyzine, Pfizer, Canada). Each chemical agent was allowed to contact the cell culture for one, three, five and ten minutes. After the treatment the cell cultures were washed with a PBS buffer and then stained with trypan blue dye. The percentage of stained and unstained cells was determined under the light microscope after one-minute exposure to the dye. After one minute of treatment 25% aluminium chloride totally damaged the cultured cells. The effect of epinephrine, aluminium sulphate and 10% aluminium chloride was less harmful. Forty percents of tetrahydrozoline-treated cells were viable even after 10 minutes. The need to preserve gingival health during and after prosthodontic treatment dictates the choice of the less harm- ful agent. Sympathomimetic vasoconstrictors were shown to be less harmful even after a prolonged period in experiment "in vitro" and are more advisable for clinical use.
Descriptors		FIBROBLASTS ALUMINUM EPINEPHRINE TETRAZOLIUM SALTS HAMSTERS TRYPAN BLUE TIME FACTORS GINGIVA DENTAL PROSTHESIS