Author/Editor		Božič, Mojca
Title		Določanje homocisteina in pojavljanje hiperhomocisteinemije pri bolnikih z vensko trombembolijsko boleznijo
Translated title		Homocysteine determination and prevalence of hyperhomocysteinemia in patients with thromboembolic disease
Type		monografija
Place		Ljubljana
Publisher		Biotehniška fakulteta
Publication year		1998
Volume		str. 43
Language		slo
Abstract		We determined total plasma homocysteine (cHCE) with two different methods, amino-acid analyser and high-performance liquid chromatography with fluorimetric detection. Because the latter method was less expensive, gave better reproducibility and the samples were stabile for a longer period, we determined cHCE in subjects with this method. We determined reference values for cHCE in 138 healthy subjects between 19 and 60 years of age (mean 40 years). CHCE concentration increased with age and was sex dependent: mean cHCE concentration in women was 17% lower then in men (6,5 micro mol/L vs. 7,8 micro mol/L, respectively; p<0,01). CHCE was significantly higher in 157 patients with venous thromboembolic disease compared to healthy subjects (8,0 micro mol/L vs. 7,2 micro mol/L, respectively; p<0,01).14% of patients had hyperhomocysteinemia defined as cHCE concentration above 95. percentile (>11,8 micro mol/L) for healthy controls. Relative risk for venous thromboembolic disease, estimated as odds ratio (adjusted for sex and total serum cholesterol), in patients with hiperhomocysteinemia was 3,2 (1,1-9,1; p<0,05). Deficiency of serum vitamin B12 and folate was not the cause of hypehomocysteinemia in our patients.
Descriptors		THROMBOPHLEBITIS HOMOCYSTEINE CHROMATOGRAPHY, HIGH PRESSURE LIQUID BLOOD COAGULATION TESTS CHOLESTEROL TRIGLYCERIDES INSULIN VITAMIN B 12 LIPOPROTEINS, HDL CHOLESTEROL LIPOPROTEINS, LDL CHOLESTEROL