Author/Editor		Šegota, Nikša; Kajba, Stanislav; Žolnir-Dovč, Manca
Title		Test pomnoževanja nukleinskih kislin iz bronhialnega izpirka v diagnostiki pljučne tuberkuloze
Translated title		The value of nucleic acid amplification test from bronchoaspirate in the diagnosis of pulmonary tuberculosis
Type		članek
Source		Zdrav Vestn
Vol. and No.		Letnik 67, št. 4
Publication year		1998
Volume		str. 229-32
Language		slo
Abstract		Background. In the study the efficacy of amplification test (AT) in bronchial aspirate (BAS) for the diagnosis of active pulmonary tuberculosis was evaluated. Methods. Between January 1995 and December 1996, 188 BAS from 185 patients, suspected of having active tuberculosis, were examined microscopically for acid fast bacilli (AFB), AT and culture. The sputum smears before fiberoptic bronchoscopy (FOB) were negative or sputum couldn't be obtained. Results. Among 185 patients (72 females and 113 males, mean age = 57.5+-15.8) who were referred for FOB, active tuberculosis was established in 31 patients (17%). AT from BAS was positive in 23 patients (sensitivity in comparison with final diagnosis is 64.5%), three patients were false positive (specificy = 98.1%). The positive predicted value of the AT from BAS was 87%. The negative predicted value of the AT from BAS was 93.2%. Conclusions. These findings suggest that the AT inbronchial aspirate is a useful and in comparison with bronchoscopic lung biopsy noninvasive method for rapid diagnosis of "smear negative" pulmonary tuberculosis. It is also important in exluding TB among patients with symptoms of other pulmonary diseases.
Summary		Izhodišča. V študiji smo ovrednotili uporabnost testa pomnoževanja nukleinskih kislin (TPNK) iz bronhialnega izpirka pri diagnostiki aktivne pljučne tuberkuloze. Metode. V obdobju od januarja 1995 do decembra 1996 smopregledali 188 bronhialnih izpirkov 185 bolnikov, pri katerih je obstajal sum, da so zboleli za pljučno tuberkulozo. Mikroskopski pregledi sputumov so bili negativni ali pa bolnik sputuma sploh ni mogel izkašljati. Bronhialne izpirke smo pregledali mikroskopsko, naredili smo TPNK inkužnine zasadili na gojišča. Rezultati. med 185 bronhoskopiranimi bolniki (72 žensk in 113 moških, povprečna starost je bila 57,5+-15,8 leta) smo opredelili aktivno pljučno tubekulozo kot končno diagnozo pri 31 (17%). TPNK iz izpirka je bil pozitiven pri 23 bolnikih (občutljivost je 64,5% v primerjavi s končno diagnozo), trije izvidi so bili lažno pozitivni (specifičnost je 98,1%). Pozitivna napovedna vrednost TPNK iz bronhialnega izpirka je bila 78%, negativna napovedna vrednost pa 93,2% glede na končno diagnozo. Zaključki. Povzamemo lahko, da je TPBK iz bronhialnega izpirka uporabna in v primerjavi z bronhoskopsko pljučno biopsijo neinvazivna metoda za hitro odkrivanje tuberkuloze pri bolnikih katerih sputumi so v direktnem razmazu negativni. Metoda je pomembna tudi za izkjučitev pljučne tubekruloze pri bolnikih z drugimi pljučnimi boleznimi.
Descriptors		TUBERCULOSIS, PULMONARY SPUTUM BRONCHOSCOPY POLYMERASE CHAIN REACTION MYCOBACTERIUM TUBERCULOSIS