Author/Editor		Petelin, Milan; Ivanuša, Teodora; Babič, Ankica; Pavlica, Zlatko; Skalerič, Uroš
Title		Regeneration of periodontal tissues after local delivery of antioxidants
Translated title		Obnova obzobnih tkiv po lokalnem dovajanju antioksidantov
Type		članek
Source		Zdrav Vestn
Vol. and No.		Letnik 67, št. Suppl 2
Publication year		1998
Volume		str. II-53-5
Language		eng
Abstract		Background. Bacteria from plaque activate gingival phagocytes to produce oxigen-derived free radicals which can cause an extensive tissue damage. The aim of our study was to evaluate the use of local administration of antioxidants in periodontal pockets, and the regeneration of periodontal tissues. Methods. After experimental periodontal breakdown followed in 15 beagle dogs, all the teeth were scaled supragingivally and animals were divided into three groups. The firt group received the liposome-encapsulated superoxide dismutase (SOD), the second group the liposome-encapsulated catalase (CAT), and the third group received both enzymes. The efficiency of the periodontal treatment by scaling and root planning, as well as the same treatment followed by the subgingival application of liposome encapsulated antioxidant enzymens was evaluated. The drugs were applied subgingivally with a syringe on a daily basis for the period of 6 weeks. Gingival index (GI), probing depth (PD), and epithelial attachment level (EAL) were measured. Radiographs were evaluated at the baseline and at the end of the experiment. An analysis of radiographs was performed by using the digital subtraction radiography (DSR). A statistical analysis of data was performed by ANOVA and Student t-test. Results. The greatest regeneration of periodontal tissues was found around the teeth by scaling and root planning followed by the SOD application. In this group a significant reduction of PD (1.1+-0.1 mm), gain of EAL (0.6+-0.1 mm), and the greatest alveolar bone apposition (10+-4.4%) was observed. Conclusion. Scaling and root planning followed by the subgingival application of the liposome encapsulated, the SOD suppressed periodontal inflammation, and stimulated the regeneration of periodontal tissues.
Summary		Izhodišča. Bakterije v zobnih oblogah aktivirajo fagocite v dlesni, da proizvedejo proste kisikove radikale. Kisikovi prosti radikali lahko poškodujejo tkivo. Namen naše raziskave je bil ugotoviti možnost lokalnega dovajanja antioksidantov v obzobne žepe in obnove obzobnih tkiv. Metode. Po eksperimentalno povzročenem vnetju obzobnih tkiv na 15 psih pasme beagle so bile okrog vseh zob odstranjene zobne obloge nad dlesnijo. Živali smo razdelili v tri skupine. prva skupina je v obzobne žepe dobivala v liposome vklučen encim superoksidno dismutazo (SOD), druga katalazo (KAT) in tretja skupina oba encima hkrati. Primerjali smo zdravljenje vnetja obzobnih tkiv z luščenjem in glajenjem zobnih korenin, luščenje in glajenje korenin ter v obzobne žepe danimi encimi. Zdravljenje je trajalo 6 tednov. Ob zob smo vsakodnevno dajali 0,2ml učinkovine. Merili smo gingivalni indeks (GI), globino sondiranja (GS) in nivo epitelijskega prirastišča (NEP). Ob začetku in na koncu zdravljenja smo naredili rentgenske posnetke zob in jih analizirali z računalniško metodo prekrivanja. Rezultate smo statistično primerjali z ANOVA in Student t-testom. Rezultati. Ob zobeh, kjer smo odstranili le zobne obloge nad dlesnijo se je GS zmanjšala le za 0,3+-0,1 mm, NEP za 0,2+-0,1 mm, GI pa se je vrnil na začetno vrednost (GI = 1,8+-0,2. Iz rentgenskih posnetkov smo ugotovili 3,6% propad čeljustne kosti. Z luščenjem in glajenjem zobnih korenin se je GS zmanjšala za 0,8+-0,1 mm, medtem ko se je NEP izboljšal enako kot pri zobeh, kjer smo odstranili le obloge nad dlesnijo. GI ob teh zobeh je ob koncu zdravljenja znašal 1,5+-0,3. Z računalniškim prekrivanjem rentgenskih slik smo ugotovili 5,4% obnovo čeljustne kosti. največja obnova obzobnih tkiv je bila ugotovljena ob zobeh, kjer smo luščili in gladili zobne korenine in v obzobne žepe dajali encim SOD.Izvleček prekinjen pri 2000 znakih.)
Descriptors		PERIODONTITIS REGENERATION SUPEROXIDE DISMUTASE CATALASE DOGS PERIODONTAL INDEX RADIOGRAPHY, DENTAL