Author/Editor		Čemažar, Maja; Miklavčič, Damijan; Serša, Gregor
Title		Intrinsic sensitivity of tumor cells to bleomycin as an indicator of tumor response to electrochemotherapy
Type		članek
Source		Jpn J Cancer Res
Vol. and No.		Letnik 89
Publication year		1998
Volume		str. 328-33
Language		eng
Abstract		Electrochemotherapy (ETC) involves the use of locally applied electric pulses to increase delivery of chemotherapeutic drugs into cells intissues. ECT with bleomycin (BLM) is a very effective local treatment, but different tumors have different response rates to ECT. The aim of our study was to compare the responsiveness of SA-1 and EAT tumors to BLM and ECT in vitro and in vivo, inorder to find possible reasons for the observed difference in response rate. The difference in sensitivity to ECT in vitro between the SA-1 and EAT cells was 10-fold and was the same as the difference in sensitivity to chronic BLM ecposure, as measured by tetrazolium-based colorimetric (MTT) assay. THis difference in sensitivity between SA-1 and EAT to ECT was also reflected in tumor cure rate. Asix-times lower dose of BLM was needed to obtain localtumor control in SA-1 than in EAT tumors. Therefore,we suggest that the difference in sensitivity to BLM and ECT predominantly reflects the difference in intrinsic sensitivity of thecells to BLM.
Descriptors		NEOPLASMS, EXPERIMENTAL ELECTRIC STIMULATION THERAPY BLEOMYCIN MICE CARCINOMA, EHRLICH TUMOR FIBROSARCOMA NEOPLASM TRANSPLANTATION SARCOMA, EXPERIMENTAL TUMOR CELLS, CULTURED