Author/Editor		Keše, Darja; Marin, Jožica
Title		Laboratorijska diagnostika klamidij
Type		članek
Source		In: Bregant L, editor. Zbornik referatov Nebakterijske okužbe v perinatologiji; 1998 maj 8-9; Ljubljana. Ljubljana: Društvo za pomoč prezgodaj rojenim otrokom,
Publication year		1998
Volume		str. 162-6
Language		slo
Abstract		It is important to make an early diagnosis of infection with Chlamydia trachomatis because of antibiotic treatment availability, it prevents transmission of bacteria in community and it prevents sequels of infection. For many years, isolation of C. trachomatis in cell culture has existed as the only test for diagnosis. But isolation of bacteria requires special equipment and stringent transport conditions. Development of the direct immunofluorescence (DIF) and enzymeimmunoasay (ELISA) for detection of chlamydia antigen in clinical specimens, made this tests more available. Unfortunately DIF and ELISA have sensitivity about 70% and thus they are not recommended for diagnosis chlamydia infections in low prevalence populations. Molecular methods, such as PCR and LCR which amplify specific sequence of nucleic acid of C. tranchomatis, are very high sensitive and specific tests. The methods of molecular biology have changed the understanding of "gold standard". Now it is submitted as expanded gold standard and it includes the isolation method and amplication of nucleic acid as well.
Descriptors		CHLAMYDIA INFECTIONS CHLAMYDIA TRACHOMATIS