Author/Editor		Rus, Boštjan; Kotnik, Tadej; Batista, Urška; Miklavčič, Damijan
Title		Določanje prevodnosti celične membrane z metodo elektrorotacije
Translated title		Determination of cell membrane conductivity by means of electrorotation
Type		članek
Source		In: Zajc B, editor. Zbornik 7. elektrotehniške in računalniške konference ERK'98. Zvezek B. Računalništvo in informatika, umetna inteligenca, robotika, razpoznavanje vzorcev, biomedicinska tehnika, močnostna elektrotehnika, didaktika, študentski članki; 1998 sep 24-26; Portorož. Ljubljana: Slovenska sekcija IEEE,
Publication year		1998
Volume		str. 391-4
Language		slo
Abstract		Exposure of biological cells to electric fields of sufficient strength leads to increased permeability of cell membrane. This phenomenon is used for introduction of different exogenous molecules into the cell. As the elctric field can be localized by electrode positioniong and shape, its effects can be focused an specific tisues or organs. In this paper, we used the electrorotational method to determine membrane conductivity of different cell lines. This method is based on the exposure of cell suspension to a rotational electric field, which influences the potential distributions of cytoplasm, membrane, and extracellular medium. Due to differences in permittivities of these regions, a phase shift between the induced potentials occurs. The phase angle between the induced potentials occurs. The phase angle between the electric field and the induced dipole moment on the cell membrane results in a torque which causes cells to rotate. Membrane conductivity is determined by measuring the dependence of the frequency of cell rotation upon the frequency of the electric field rotation. This dependence is characterized by two peaks that occur at the so-called characteristic frequencies. The first characteristic frequency allows to calcute the membrane conductivity, if values of other electrical and geometrical parameters of the model are known. We determined membrane conductivities of five cell lines, and repeated the measurement on several days. Thus we were able to check the reproducibility of the results within the same cell line, and to compare the conductivities of different cell lines. Comared to the published data, the calculated conductivities are within the same order of magnitude, but in general somewhat higher.(Abstract truncated at 2000 characters.)
Descriptors		CELL MEMBRANE PERMEABILITY CELL LINE ELECTRIC CONDUCTIVITY ELECTROMAGNETIC FIELDS CELL TRANSFORMATION, NEOPLASTIC