Author/Editor		Keše, Darja; Sočan, Maja; Marin, Jožica; Eberl-Gregorič, Edita
Title		Dokazovanje DNA Legionella v urinu z verižno reakcijo s polimerazo
Translated title		Detection of Legionella DNA in urine samples by polymerase chain reaction
Type		članek
Source		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Publication year		1998
Volume		str. 14-7
Language		slo
Abstract		Laboratory diagnosis of legionella infection could be difficult. Existing laboratory tests have lesser sensitivity or to enable only a retrospective diagnosis. Therefore, we wished to evaluate polymerase chain reaction (PCR) to detect legionella DNA in urine samples from patients. One hundred hospitalized patients (older than 15 years) with community-acquired pneumonia were included in the study. We used the PCR with highly specific biotinylated primers complementary to conserved regions of the 5S rRNA gene to detect Legionella DNA and biotinylated primers complementary to seuence of the mip gene to detect L. pneumophila DNA. Urine samples were also tested for presence of a heat stable soluble antigen of L. pneumophila SG1 by enzymeimmunoassay (EIA). Paired sera were tested against antigens of L. pneumophila SG1-7 and L. micdadei with use of the indirect immunofluorescence test (IIF). Positive titer of antibodies was detected in 14 patients; whereas urinary detection was positive in 9 samples. PCR was positive in 22 urine samples. The sensitivity of the PCR assay in urine samples was 87.5%, while urinary antigen test had lower degree of sensitivity ((56.3%). The specificity of PCR was 92.8% comparing with EIA test where the specificity was 100%. Our results showed that detection of Legionella DNA in urine samples by PCR would be useful tool for the diagnosis of legionella pneumonia.
Descriptors		LEGIONELLA POLYMERASE CHAIN REACTION URINE LEGIONNAIRES' DISEASE LEGIONELLOSIS IMMUNOENZYME TECHNIQUES FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT