Author/Editor		Gasparič, A; Dular, T; Grošel, A; Radež, R; Sladič, G; Pečnik, T; Radež, I; Pelko, M; Mandič-Mulec, I
Title		Bacitracin - product of peptide synthetase
Type		članek
Source		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Publication year		1998
Volume		str. 61-3
Language		eng
Abstract		A vast array of natural peptides diverse in their structure are produced by microorganisms living in different aquatic and terrestrial environments, are not gene encoded but are synthetized nonribosomally on peptide synthetases. The rate of peptide antibiotic bacitracin biosynthesis positively correlates with the level of the alkaline protease production. To define some positivety acting factors in terms of bacitracin production we performed an experiment expressing genes involved in the process of sporulation and compotence (B. subtilis): degU on pBD1834, sinIR on pIS74 and sinR on pIS119 in B. licheniformis. Bacterial clones were analyzed for the proteolytic activity and bacitracin production. In 9 clones (2 x pBD1834, 2 x pIS74 in 5 x pIS119) high rate of the peptide antibiotic bacitracin production was determined.
Descriptors		BACITRACIN CLONE CELLS ANTIBIOTICS, PEPTIDE PEPTIDE SYNTHETASES BACILLUS GENE EXPRESSION REGULATION, BACTERIAL