Author/Editor		Ocepek, M; Posedi, J
Title		Primernost gojišča BBL MGIT za uporabo v veterinarskem mikrobakteriološkem laboratoriju
Translated title		Suitability of BBL MGIT growth medium in veterinary mycobacteriology
Type		članek
Source		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Publication year		1998
Volume		str. 160-3
Language		slo
Abstract		We have compared BBL MGIT growth medium (Becton Dickinson) with 4 conventional solid media (Middlebrook 7H10, Stonebrink, IUT - International Unit Against Tuberculosis medium with pyruvate and IUT medium with glycerol). Specimens of organs obtained from 345 animals were decontaminated with N-acetyl-L-cysteine by the standard method. The mycobacteria were isolated from 175 specimens. MGIT was positive from 129 (73.7%) specimens, IUT-P medium from 117 (66.9%) specimens, Middlebrook 7H19 medium from 106 (60.6%) specimens, IUT-G medium from 106 (60.6%) specimens and Stonebrink from 97 (55.4%) specimens. The isolation of mycobacteria succeeded from 26 samples only on MGIT in 7 samples only on UIT-P, 4 samples were isolated only on IUT-G, additional 4 samples only on 7H10 medium and 1 sample only on Stonebrik medium. The mean time for detection of mycobacteria in MGIT was 17 days (range 3 - 68 days), in Stonebrink medium, 25 days (range 10 - 56 days), in Middlebrook 7H10 medium 25 days (range 7 - 63 days), in IUT-P medium 26 days (range 5 - 68 days) and in IUT-G medium 30 days (range 5 - 64). In average, using MGIT medium reduced the time for detection for 9 days. Eithout MGIT the results from 26 (14.9%) specimens would de false negative.
Descriptors		MYCOBACTERIUM CULTURE MEDIA MYCOBACTERIUM INFECTIONS