Author/Editor		Ocepek, M; Zabavnik-Piano, Jelka; Pislak, Metka
Title		Ugotavljanje Mycobacterium avium subsp. paratuberculosis v tkivih prežvekovalcev z metodami PCR
Translated title		Detection of Mycobacterium avium subsp. paratuberculosis in the tissue of ruminants by methods of PCR
Type		članek
Source		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Publication year		1998
Volume		str. 164-7
Language		slo
Abstract		Paratuberculosis is a chronic infectious disease caused by Mycobacterium avium subsp. paratuberculosis. To diagnose the disease the isolation and determination of the causing agent is necessary, which can take several months due to very slow growth of mycobacteria. We are introducing the method of polymerase chain reaction - PCR to detect the DNA of M.avium subsp. paratuberculosis which shortens the procedure to 2 to 3 days. In our study we have analysed the samples of intestines and mesenterial lymphatic glands obtained from eight serological positive ruminants, included wwere also positive and negative control and the control to determine the specificity of the reaction. Oligonucleotide primers P90 and P91 were used to amplify the nucleotide sequence of the insertion sequence IS900, characteristic for M.avium subsp. paratuberculosis. The products of PCR reaction were further amplified by nested PCR. The product of nested PCR was labelled and used as a probe in the dot blot hybridisation. The results of our study indicate that PCR could be used to detect M. avium subsp. paratuberculosis in the tissues of domestic animals.
Descriptors		MYCOBACTERIUM AVIUM MYCOBACTERIUM PARATUBERCULOSIS PARATUBERCULOSIS CATTLE SHEEP POLYMERASE CHAIN REACTION