Author/Editor		Krt, Brane
Title		Encimskoimunski test za ugotavljanje toksina epsilon Clostridium perfingens
Translated title		ELISA for detection of Clostridium perfingens epsilon toxin
Type		članek
Source		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Publication year		1998
Volume		str. 168-71
Language		slo
Abstract		Enzyme immunoassay as an alternative test to the biological assay for the detection of C. perfrigens epsilon toxin was developed. Microplate was coated with standard horse epsilon antitoxin. After overnight incubation the supernatants of reference C. perfringens (types A, B and C) cultures were added. Epsilon toxoid and culture medium were used as positive and negative controls, respectively. In the next step monoclonal antibodies against epsilon toxin and after them anti-mouse IgG-peroxidase conjugate were added. The reaction was strongly positive in C. perfringens type B and negative in types A and C. In the second variant the microplate was coated with monoclonal antibodies against epsilon toxin. The standard horse epsilon antitoxin was used for the detection, followed by anti-horse conjugate. This time the differences between tested toxins were lower. For the complete evaluation of ELISA more isolates of C. perfringens shoud be tested and the comparison with the results of biological assay (which is still the conventional test) should be done. However, when compared biological assay in mice or guinea pigs ELISA is more rapid and cheaper method with higher testing capacity. The use of this method reduces the rewuirement for experimental animals in laboratory diagnosi.
Descriptors		CLOSTRIDIUM PERFRINGENS BACTERIAL TOXINS ENZYME-LINKED IMMUNOSORBENT ASSAY