Author/Editor     Prinčič, Alenka; Mahne, Ivan; Megušar, France; Laanbroek, Hendrikus J
Title     Strukturne spremembe nitrifikacijskih združb pri selektivnih dejavnikih okolja: DGGE (denaturation gradient gel electrophoresis) in filogenetska analiza
Translated title     Response of nitrifying communities to selective environmental conditions: DGGE (denaturation gradient gel electrophoresis) and phylogenetic analysis
Type     članek
Source     In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Publication year     1998
Volume     str. 217-20
Language     slo
Abstract     Changes in nitrifying community structure in response to different prevailing ammonium concentrations (50, 500, 1000, 3000 mg/l), pH values (6.0, 7.0, 8.2) and oxygen levels (1%, 7%, 21%) were studied in experimental reactors containing bacterial community from a waste water. Denaturing gradient del electrophoresis (DGGE) of PCR products obtained using ammonia oxidizer specific rDNA primers revealed large community structure shift induced by high ammonium levels and was reversible once the ammonium was returned to its original low level. The changes in community structure brought about by both pH extremes, however were irreversible. Ribosomal DNA sequence analysis revealed that high ammonium and deviating pH environments stimulated growth of ammonia oxidizer strains that are substantially different from their nearest relatives, Nitrosomonas eutropha C-91 in Nitrosomonas europaea.
Descriptors     NITROSOMONAS
NITROSATION
AMMONIUM COMPOUNDS
EVOLUTION, MOLECULAR
ELECTROPHORESIS
HYDROGEN-ION CONCENTRATION
DNA, RIBOSOMAL
OXYGEN