Author/Editor		Kuret, J; Poljšak-Prijatelj, M; Drinovec, B; Šest, I; Pfeiffer, S
Title		Razmnoževanje virusa ošpic v celični kulturi CV 1
Translated title		Replication of measles virus cell culture CV 1
Type		članek
Source		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Publication year		1998
Volume		str. 416-9
Language		slo
Abstract		Measles virus belongs to the family Paramyxoviridae and is causative agent of the disease. Although the measles today do not represent a major medical problem, occasionally problems still occur. We were interested in time interval needs for one replication cycle of the virus within the cell. For the study we employed immunofluorescence and electron microscopical methods. As an experimental model, CV1 cell culture, infected with measles virus, strain Edmonston, was used. Infected cell cultures were examined for virus shedding by immunofluorescence test every two hours, starting 16 hours p.i. and concluding 24 hours p.i., to determine how long after the infection, massive virus budding occurs. We found that between 20 - 22 hours p.i. the budding is most intensive. For electron microscopical examinations we used cell 21 hours p.i.. Cells were aldhyde fixed and viral antigens, present in cell membrane were labelled by immunogold technique. Subsequently, cells were dehydrated by progressive lowering of temperature, embedded in HM20 and polymerized under UV light at - 50 degrees C (223 K). Ultrathin sections were examined in transmission electron microscope Jeol JEM1200 EX II.
Descriptors		MEASLES VIRUS VIRUS REPLICATION CELL LINE