Author/Editor		Škoberne, M; Malovrh, T; Matis, M; Ružić-Sabljić, E; Kotnik, V
Title		Ultrazvočno dezintegrirana Borrelia afzelii osamljena iz miši je kemotaktični dejavnik za polimorfonuklearne fagocitne celice
Translated title		Sonificated Borrelia afzelii isolated from a mouse is chemotactic for polymorphonuclear phagocytes
Type		članek
Source		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Publication year		1998
Volume		str. 467-70
Language		slo
Abstract		Lyme disease is caused by infection with Borrelia afzelii. Many experiments have shown that neutrophils are one of the most important mechanisms for eliminating Borrelia and that resistance to phagocytic elimination correlates with infectivity of B. afzelii isolates. We used C5a to stimulate chemotaxis of polymorphonuclear granulocytes and measured potential inhibitory role of Borrelia antigens. We exposed human neutrophils to a mixture of a strong chemoattractant C5a and ultrasonically disintegrated B. afzelii isolated from an infected mouse. The ultrasonically disintegrated B. afzelii did not impair chemotaxis of neutrophils stimulated by C5a. In contrast, when neutrophils were exposed only to B. afzelii antigens they also showed some chemotactic activity compared to negative control.
Descriptors		BORRELIA ULTRASONICS NEUTROPHILS COMPLEMENT 5A CHEMOTAXIS, LEUKOCYTE MICE ANTIGENS, BACTERIAL