Author/Editor		Kotnik, Antonija; Škoberne, Mojca; Malovrh, Tadej; Kotnik, Vladimir
Title		Potek s HSV-1 sprožene apoptoze v celicah HEp-2
Translated title		HSV-1 induced apoptosis in Hep-2 cell line
Type		članek
Source		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Publication year		1998
Volume		str. 475-8
Language		slo
Abstract		HSV-1 infection of HEp-2 cells in vitro result in specific changes of target cell monolayer. Two days after the virus inoculation the HEp-2 cells monolayer appears as moth swallowed wool carpet. By generally accepted opinion HEp-2 cell die because of necrosis. In our experiments we show that at least some of the cells deteriorate because of the apoptosis one or two days after the infection. The apoptosis rate was established with detection of mono and oligonucleosomes (180bp) in cytoplasmatic fraction of lysed HSV-1 infected HEp-2 cells. The nucleosomes were fixed to the bottom of plastic reagent vessel using monoclonal antihistone antibodies detecting epitopes H2A, H2B, H3 and H4. In the next step the immobilized nucleosomes were coated with specific anti-DNA POD labeled antibodies. Quanitity of the bound enzyme was determined measuring amount of disintegrated ABTS (2,2'-anzino'di'*3-ethylbenzthiazoline sulfonate (6))). We found that the apoptosis of GSV-1 infected HEp-2 cells depends on quantity of virus inoculum and time elapsed from the infection.
Descriptors		CELLS, CULTURED SIMPLEXVIRUS APOPTOSIS HERPESVIRUS 1, SUID ENZYME-LINKED IMMUNOSORBENT ASSAY PROTO-ONCOGENE PROTEINS C-BCL-2