Author/Editor		Mueller-Premru, Manica; Gubina, Marija; Stražiščar, Tina
Title		Tipizacija bakterije Pseudomonas aeruginosa s pulzno elektroforezo
Type		članek
Source		In: Bole-Hribovšek V, Ocepek M, Klun N, editors. Zbornik s programom 2. kongres slovenskih mikrobiologov z mednarodno udeležbo; 1998 sep 27-30; Portorož. Ljubljana: Slovensko mikrobiološko društvo,
Publication year		1998
Volume		str. 503-6
Language		slo
Abstract		In this study we wanted to determine whether clinical isolates of Pseudomononas (P.) aeruginosa differ inside the same serotype in antibiotic susceptibility and genotype. Antibiotic susceptibility testing and pulsed-field gel electrophoresis (PFGE) of chromosomal DNA were performed on 16 isolates in 13 patients. Eleven isolates belonged to serotype O 11 and five to serotype O 6. Serotype O 11 isolates were collected fromfive hospital departments, but serotype O 6 isolates from a single department. Nine serotype O 11 isolates were multiresistant while two were not. All serotype O 6 isolates were susceptible to antibiotics. Sixteen P. aeruginosa isolates had five different antibiotic susceptibility patterns and nine PFGE patterns, representing types or clones. Serotype O 11 isolates Serotype O 11 isolates had four susceptibility and PFGE patterns, but serotype O 6 isolates had one antibiotic susceptibility pattern and five PFGE patterns. PFGE patterns clearly separated between serotype O 11 and O 6 isolates. Among multiresistant serotype O 11 isolates PFGE patterns were less diverse than among susceptible O 6 and O 11 isolates. From our results we can conclude that clinical isolates of P. aeruginosa differ inside serotype by antibiotic susceptibility and by genotype. Genotyping is more discriminatory than serotyping and antibiotic susceptibility testing.
Descriptors		PSEUDOMONAS AERUGINOSA GENOTYPE BACTERIAL TYPING TECHNIQUES ELECTROPHORESIS, GEL, PULSED-FIELD