Author/Editor		Kovač, M; Luskovec, M; Vilhar, B; Ravnikar, M
Title		Peroxidase activity during rooting of potato stem nodes on medium with and without jasmonic acid
Translated title		Peroksidazna aktivnost med koreninjenjem nodijskih izsečkov stebla pri krompiru na gojišču z in brez jasmonske kisline
Type		članek
Source		Acta Biol Slov
Vol. and No.		Letnik 41, št. 4
Publication year		1997
Volume		str. 61-7
Language		eng
Abstract		In order to examine whether a peak of peroxidase activity is a marker of root initiation in potato as it in several other plant species we measured it during rooting in potato stem node culture. Furthermore, we studied the effects of jasmonic acid (JA) on peroxidase activity in relation to root development. Peroxidase activity, total protein, fresh and dry weight wer measured in single-node stem cuttings of potato (Solanum tuberosum L. cv. Ulster Sceptre) grown on the medium without (control) or with 1 micro M JA. During the first 13 hours of culture, the specific peroxidase activity (per mg dry weight and per mg protein) was low in both treatments, and then increased steadily during later stages of the of the experiment. A peak of peroxidase activity was not observed in the interval between excision of cuttings and emergence of the first roots on day 3. Compared to the control treatment, JA did not affect root emergence, percentage dry weight and specific peroxidase activity per mg dry weight. However, the amount of protein measured after root emergence was slightly lower in the JA-treatment compared to the control while specific peroxidase activity per mg protein was higher. Hence, although JA markedly affects growth and morphology of potato roots in culture, it does not alter peroxidase activity significantly in the interval between excision of cuttings and emergence of roots.
Descriptors		POTATOES PLANT GROWTH REGULATORS PLANT ROOTS PEROXIDASES PLANT STEMS PLANT PROTEINS