Author/Editor		Čemažar, Maja; Jarm, Tomaž; Miklavčič, Damijan; Maček-Lebar, Alenka; Ihan, Alojz; Kopitar, Nataša A; Serša, Gregor
Title		Effect of electronic-field intensity on electropermeabilization and electrosensitivity of various tumor-cell lines in vitro
Type		članek
Source		Electro Magnetobiol
Vol. and No.		Letnik 17, št. 2
Publication year		1998
Volume		str. 263-72
Language		eng
Abstract		Electropermeabilization (electroporation) is a technique widely used to introduce various membrane-impermeable molecules into cells in vitro or in vivo. In this study we determined the effect of different electric-field intensities on electropermeabilization and electrosensitivity of a variety of tumor-cell lines in vitro. For this purpose we used two assays: propidium iodide uptake for measurement of cell electropermeabilization, and the clonogenic or MTT assay for determination of electrosensitivity. Our results showed that electropermeabilization of almost all cell lines tested occurred at 600 V/cm. In contrast, a marked difference in electrosensitivity existed among these cell lines. Our results could be of great importance for pharmacological and biochemical studies in vitro, and for prediction and determinatin of tuor response in vivo to electropermeabilization combined with chemotherapeutic drugs (electrochemotherapy) and gene therapy.
Descriptors		TUMOR CELLS, CULTURED ELECTROPORATION CELL SURVIVAL CELL MEMBRANE PERMEABILITY ELECTROMAGNETIC FIELDS