Author/Editor		Kordiš, Dušan; Gubenšek, Franc
Title		The Bov-B lines found in Vipera ammodytes toxic PLA2 genes are widespread insnake genomes
Type		članek
Source		Toxicon
Vol. and No.		Letnik 36, št. 11
Publication year		1998
Volume		str. 1585-90
Language		eng
Abstract		The Bov-B LINEs found in Vipera ammodytes toxic PLA2 genes are widespread in snake genomes. Toxicon 36. 1585-1590. 1998. -In the fourth intron of two toxic Vipera ammodytes PLA2 genes a Ruminantia specific 5'-truncated Bov-B LINE element was identified. Southern blot analysis of Bov-B LINE distribution in vertebrates shows that, apart from the Ruminantia, it is limited to Viperidae snakes (V. ammodytes, Vipera palaestinae, Echis coloratus, Bothrops alternatus, Trimeresurus flavoviridis and Trimeresurus gramineus). The copy number of the 3' end of Bov-B LINE in the V. ammodytes genome is between 62000 and 75000. At orthologous positions in other snake PLA2 genes the Bov-B LINE element is absent, indicating that its retrotransposition in the V.ammodytes PLA2 gene locus has occurred quite recently, about 5 Myr ago. The amplification of Bov-B LINEs in snakes may have occured before the divergence of the Viperinae and Crotalinae subfamilies. Due to its wide distribution in Viperidae snakes it should be a valuable phylogenetic marker. The neighbour-joining phylogenetic tree shows two clusters of truncated Bov-B LINE, a Bovidae and a snake cluster, indicating an early horizontal transfer of this transposable element.
Descriptors		VIPERIDAE PHOSPHOLIPASES A VIPER VENOMS PHYLOGENY GENOME INTRONS BASE SEQUENCE RUMINANTS