Author/Editor		Sepčić, Kristina; Marcel, Veronique; Klaebe, Alain; Turk, Tom; Šuput, Dušan; Fournier, Didier
Title		Inhibition of acetylcholinesterase by an alkylpyridinium polymer from the marine sponge, Reniera sarai
Type		članek
Source		Biochim Biophys Acta
Vol. and No.		Letnik 1387
Publication year		1998
Volume		str. 217-25
Language		eng
Abstract		Large polymeric 3-alkylpyridinium salts have been isolated from the marine sponge Reniera sarai. They are composed of N-butyl(3-butylpyridinium) repeating subunits, polymerized head-to-tail, and exist as a mixture of two main polymers with molecular weights without counterion of about 5520 and 18900. The monomer analogue of the inhibitor, N-butyl-3-butylpyridinium iodide has been synthesized. This molecule shows mixed reversible inhibition of acetylcholinesterase. The polymers also act as acetylcholinesterase inhibitors and show an unusual inhibition pattern. We tentatively describe it as quick initial reversible binding, followed by slow binding or irreversible inhibition of the enzyme. This kinetics suggests that there are several affinity binding sites on the acetylcholinesterase molecule where the polymer can bind. The first binding favors binding to other sites which leads to an apparently irreversibly linked enzyme-inhibitor complex.
Descriptors		PORIFERA ACETYLCHOLINESTERASE PYRIDINIUM COMPOUNDS CHOLINESTERASE INHIBITORS POLYMERS NITROBENZOATES BINDING SITES SEAWATER