Author/Editor		Župunski, Vera
Title		Multigenska družina BPTI homologov pri modrasu
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1998
Volume		str. 91
Language		slo
Abstract		Members of the Kunitz type bovine pancreatic trypsin inhibitor (BPTI) family are widespread throughout the animal kingdom. Venom of snakes from the families Elapidae and Viperidae also contains BPTI homologues whose amino acid sequences show up to 50% homology and 3-D structures very similar to BPTI. However, some of the BPTI homologues are functionally different and show neurotoxic activity arising from the amino acid substitions. Three different BPTI homologues have been isolated from the venom of the longnosed viper (Vipera ammodytes). Genomic and cDNA libraries were screened with the PCR amplified coding region of trypsin inhibitor. We isolated five positive genomic clones and a large number of cDNA clones. From the nature of residue 15 (BPTI numbering) four out of five genomic clones code for chymotrypsin inhibitors and one codes for trypsin inhibitor. The structural organisation of the genes was deduced by comparing gene coding regions and related cDNAs. Two of the positive cDNA clones were sequenced but both of them have truncated 5' ends. The trypsininhibitor cDNA is 378 bp long, coding for 17 out of 24 amino acids of the signal peptide and 66 amino acids of the mature protein. Chymotrypsin inhibitor cDNA is 379 bp long, coding for 19 amino acids of the signal peptide, 65 amino acids of the mature protein and 4 C-terminal amino acids of precursos inhibitor. Due to the truncated 5' ends ofisolated cDNA, it is not possible to detemine the complete gene structure. However, from the comparison of cDNAs and genes we have deduced the exact boundaries between exons, introns and 3' flanking regions. Only the boundary between the 5' flanking region and the first exon remains undefined.(Abstract truncated at 2000 characters)
Descriptors		VIPER VENOMS SERINE PROTEINASE INHIBITORS TRYPSIN INHIBITORS DNA, COMPLEMENTARY BASE SEQUENCE VIPERIDAE INTRONS CHYMOTRYPSIN