| Author/Editor | Košnik, Mitja | |
| Title | Imunski odziv v začetni fazi imunoterapije s strupi kožekrilcev: vloga celične imunosti pri vzpostavitvi specifične tolerance za alergen v začetni fazi imunoterapije s strupi kožekrilcev | |
| Translated title | Immune response in the early phase of immunotherapy with Hymenoptera venoms: role of cellular immunity in establishment of specific tolerance to allergen in early phase of immunotherapy with Hymenoptera venom | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 1998 | |
| Volume | str. 105 | |
| Language | slo | |
| Abstract | Background: Mechanism of immune tolerance during specific immunotherapy (SIT) with subcutaneous injections of allergen (Ag), particularly mechanism of immediate tolerance, is still unclear. Methods: We compared function of mast cells, basophils, lymphocyte B and T in 11 patients with venom allergy before SIT (DAY 1 ), after rush phase (DAY 4), before first maintenance dose (DAY 14) and after 6 months of SIT (DAY 180) and with 11 healthy volunteers. At each time point we performed skin prick tests with codeine and intradermal tests with wasp and honey bee venom. Blood was taken for determination of specifc IgE and IgG4, in vitro stimulation of leukocytes and measurement of released sulphidoleukotrienes (sLT) (CAST ELISA), and stimulation of mononuclear cells with Ag and mitogens (either with PMA and ionomycine or anti-CD3 antibodies) and measurement of released IL-2, IL-4, IL-5, IFN-gamma and soluble IL-2R. Results: During rush immunotherapy skin response to Ag used for SIT was diminished (DAY 1: 8,2+-1,7 mm, DAY 4: 7,2+-1,3 mm, p<0,05) but later it returned to pretreatment level. There were no changes in skin reactivity to codeine and histamine. During rush SIT there was a nonspecific decrease of sensitivity of basophils to stimuli acting through Fcepsilon-RI (Ag used for SIT, Ag not used for SIT, anti-Fcepsilon-RI antibodies), but not to ionomycine. There was no decrease in slgE but sIgG4 increased signifcantly after only rush SIT. After stimulation with Ag MNC of patients released 86+-133 pg/ml IFN-gamma and 19,7+-20,1 pg/ml IL-4 (Th2 response), MNCs of healthy controls released 184+-116 pg/ml IFN-gamma and 4,8+-8 pg/ml IL-4 (Th1 response). Spontaneous release of IFN-gamma in cultures of MNC of patients increased after rush SIT (DAY 1: 25+-31 pg/ml, DAY 14 241+-281 pg/ml, p<0,05). (Abstract truncated at 2000 characters) | |
| Descriptors | BEE VENOMS WASP VENOMS DESENSITIZATION, IMMUNOLOGIC HYPERSENSITIVITY, IMMEDIATE SKIN TESTS CODEINE HISTAMINE ALLERGENS INTRADERMAL TESTS IGE IGG BASOPHILS MAST CELLS LYMPHOCYTE TRANSFORMATION CYTOKINES TETRADECANOYLPHORBOL ACETATE IONOMYCIN INTERLEUKINS INTERFERON TYPE II LYMPHOCYTE COUNT |