Author/Editor		Garaj-Vrhovac, Vera; Kopjar, Nevenka
Title		Micronuclei in cytokinesis-blocked lymphocytes as an index of occupational exposure to antineoplastic drugs
Translated title		Mikronukleus test pri osebah s poklicno izpostavljenostjo citostatikom
Type		članek
Source		Radiol Oncol
Vol. and No.		Letnik 32, št. 4
Publication year		1998
Volume		str. 385-92
Language		eng
Abstract		In order to investigate possible DNA damaging effects of antineoplastic drugs, the micronucleus assay combined with Giemsa, DAPI and silver staining was performed. Blood samples were taken from nurses working without adequate protection in oncological department on preparing and administration of different antineoplastic drugs. Lymphocytes were cultivated in vitro at 37 degrees C. To prevent cytokinesis, at 44th cytochalasine-B at a final concentration of 3 micro g/ml was added. The results obtained indicate statistically significant increases in the total number of micronuclei in the exposed subjects compared to controls. DAPI staining has revealed signal-positive and signal-negative micronuclei while silver staining has revealed Ag-NOR+ and Ag-NOR- micronuclei. Compared to controls, the number of signal-positive and Ag-NOR+ micronuclei in the exposed subjects were increased, indicating a greater susceptibility of particular chromosomes to damage caused by antineopalstic agents.
Summary		Avtorji so s pomočjo mikronukleus testa v kombinaciji z različnimi tehnikami barvanja (Giemsa, DAPI in srebro) proučevali možne poškodbe DNK zaradi delovanja citostatikov. Krvne vzorce za preiskave so jemali medicinskim sestram, ki so na onkoloških oddelkih brez ustrezne zaščite opravljale delo v zvezi s pripravo in aplikacijo različnih citostatikov. Limfocite so gojili in vitro pri temperaturi 37 degrees C. Za preprečitev citokineze so po 44 urah dodali citohalazin B v končni koncentraciji 3 micro g/ml. Dobljeni rezultati kažejo statistično značilen porast skupnega števila mikronukleusov pri izpostavljeni skupini v primerjavi s kontrolno skupino. Z DAPI tehniko so prikazali signal-pozitivne in signal-negativne mikronukleuse, medtem ko je tehnika bavanja s srebrom razkrila Ag-NOR-pozitivne in Ag-NOR-negativne mikronukleuse. V primerjavi s kontrolno skupino je bilo število signal-pozitivnih in Ag-NOR-pozitivnih nukleusov pri izpostavljenih osebah zvišano, kar kaže, da so nekateri kromosomi bolj dovzetni za poškodbe, ki jih povzročajo citostatiki.
Descriptors		ANTINEOPLASTIC AGENTS OCCUPATIONAL EXPOSURE MICRONUCLEUS TESTS LYMPHOCYTES