Author/Editor		Šepič, E; Bricelj, M; Leskovšek, H
Title		Degradation of fluoranthene by Pasteurella sp. IFA and Mycobacterium sp. PYR-1: isolation and identification of metabolites
Type		članek
Source		J Appl Microbiol
Vol. and No.		Letnik 85
Publication year		1998
Volume		str. 746-54
Language		eng
Abstract		The findings from a biodergradability study of fluoranthene using two pure bacterial strains, Pasteurella sp. IFA (B-2) and Mycobacterium sp. PYR-1 (AM) are reported. of total fluoranthene, 24% (B-2) and 46% (AM) was biodegraded in an aqueous medium during 14 d of incubation at room temperature. During this period the bacteria were capable of mineralizing approximately two-thirds (B-2) and four-fifths (AM) of biodergraded fluoranthene to CO2, while one-third (B-2) and one-fifth (AM) of the original fluoranthene remained as stable metabolic products. These metabolites were isolated using liquid-liquid extraction and identified using gas chromatography - mass spectrometry (GC-MS) and derivatization techniques. Two metabolites (9-fluorenone-1-carboxylic acid and 9-fluorenone) were identified by GC-MS directly, while the metabolites 9-fluorenone-1-carboxylic acid, 9-hydroxyfluorene, 9-hydroxy-1-fluorene-carboxylic acid, 2-carboxybenzaldehyde, benzoic acid and phenylacetic acid were determined in their derivatized forms. From the identified metabolites, a fluoranthene biodegradation pathway was proposed for Pasteurella sp. IFA.
Descriptors		PASTEURELLA MYCOBACTERIUM BIODEGRADATION POLYCYCLIC HYDROCARBONS, AROMATIC FLUORENES CHROMATOGRAPHY, GAS SPECTRUM ANALYSIS, MASS