| Author/Editor | Coer, Andrej | |
| Title | Proliferation and proliferation markers | |
| Type | članek | |
| Source | In: Pajer Z, Štiblar-Martinčič D, editors. International symposium on cardiovascular diseases. Proceedings of the 29th memorial meeting devoted to prof. dr. Janez Plečnik; 1998 Dec 3-5; Ljubljana. Ljubljana: Medical faculty, Institute of histology and embryology, | |
| Publication year | 1998 | |
| Volume | str. 363-70 | |
| Language | eng | |
| Abstract | Cell proliferation is one of the most fundamental of biological processes. Cardiac myocytes were considered to be terminally differentiated cells that no longer possess the capacity of undergoing DNA synthesis and mitotic division. In recent years, numerous quantitative morphological studies have provided strong supportive evidence that ventricular myocytes are not terminally differentiated cells, and myocyte cellular hyperplasia may constitute are not terminally differentiated cells, and myocyte cellular hyperplasia may constiute a significant component of ventricular remodelling in the adult human heart. Different methods for myocyte proliferation assessment have been used in those studies. This brief overview is intended to illustrate the advantages and disadvantages of the better known methods for assessing cellular proliferation in histopathological material of human hearts. An absolute requirement for pathologists is to have a clear understainding of the biology of proliferation markers. A single proliferation market can hardly be interpreted independently of others. Three types of markers are necesary to describe cell population kinetics: one measures the growth fraction (Ki-67, MIB-1,...), the second evaluates the cell cycle speed inarbitrary units (Ag-NORs) and the last assesses the S phase cells occurrence frequency (BrdU, PCNA,...) or mitosis frequency. Combining the three types of kinetic parameters may provide insight into still poorly understand myocyte proliferation. | |
| Descriptors | CELL DIVISION MITOTIC INDEX NUCLEOLUS ORGANIZER REGION CARDIOMYOPATHY, CONGESTIVE CELL CYCLE THYMIDINE BROMODEOXYURIDINE IMMUNOHISTOCHEMISTRY PROLIFERATING CELL NUCLEAR ANTIGEN |