| Author/Editor | Čandek-Potokar, Marjeta; Uršič, M; Fazarinc, G | |
| Title | Vrednosti pH1 najdaljše hrbtne mišice (m. longissimus dorsi) v odvisnosti od njene histokemične zgradbe pri za stresni sindrom (PSS) nedovzetnih prašičih | |
| Translated title | Histochemical profile of longissimus dorsi muscle as related to pH1 in porcine stress syndrome (PSS) resistant pigs | |
| Type | članek | |
| Source | Zb Vet Fak Univ Ljublj | |
| Vol. and No. | Letnik 35, št. 1-2 | |
| Publication year | 1998 | |
| Volume | str. 19-26 | |
| Language | slo | |
| Abstract | The influence of muscle fibre type composition on the rate of pH fal (pH1) in longissimus dorsi muscle was investigated in 39 castrated pigs (Swedish Landrace x Large White) x Duroc), resistant to the porcine stress syndrome (PSS). They were all reared on the same farm and were slaughtered in the same commercial abattoir under similar conditions.. The measurement of pH1 was taken 45 minutes after slaughter in lomgissimus dorsi muscle at the level of the last rib. Muscle samples for the histochemical analysis were taken at the same anatomical location. Cryostat sections (10(m) were stained using a combined method of successive staining for the succinate dehydrogenase (SDH) activity and immunohistochemical detection of last myosin heavy chains on the same cryostat section. Myofibres were classified as slow, red (SSDH+), fast red (FSDH+) and fast white (FSDH-) fibres. pH1 values inferior or equal to 5.9 were considered as condition leading to PSE meat which actually occurred in 7 (17.9%) cases. A negative relationship was found between pH1 and the percentage of FSDH- fibres (r = -0.50, P<0.01), whereas it was positive with the percentage of SDH positive fibres (SSDH+ and FSDH+; r = 0.30, P<0.10; r = 0.40, P<0.01, respectively). On the basis of our results we concluded that a higher percentage of glycolytic fibres increase the rate of post-mortem pH fall. In light of the present study it is clear, that the problem of PSE meat will not be entirely solved only by eliminating pigs which are carriers of the mutated RYR 1 gene. In particular, management of the pigs prior to slaughter will still have to be improved to prevent fast post-mortem metabolism in muscle. | |
| Descriptors | MUSCLE, SKELETAL MYOFIBRILS HYDROGEN-ION CONCENTRATION SWINE STRESS MYOSIN HEAVY CHAINS SUCCINATE DEHYDROGENASE |