Author/Editor		Zajc, Irena; Sampson, J
Title		Preverjanje starševstva pri čistokrvnih pasmah domačih psov s pomočjo raznolikih mikrosatelitov
Translated title		Parentage testing in pure breeds of domestic doug using polymorphic microsatellites
Type		članek
Source		Zb Vet Fak Univ Ljublj
Vol. and No.		Letnik 35, št. 1-2
Publication year		1998
Volume		str. 27-34
Language		slo
Abstract		Variable canine microsatellites, a class of repetitive DNA sequences, have been used successfully in applications as diverse as population studies, studies of evolutionary relationships and individual identification. In parentage testing however, if the access to all the samples is denied, only the definite exclusion of wrongly "accused" parents is possible. Without the population statistics on the frequency of microsatellite alleles no reasonable probability to patternity or parentage could be given. In the present study the utility of microsatellites for parentage testing is demonstrated in two cases. For Greyhounds, Labradors and German shepherds the parentage indeces, essential to estimate the probability of parentage are also given. Highly polymorphic microsatellite loci that are common to all three breeds are the best candidates for the panel of universal loci that would be useful for parentage testing in any dog breed. Exclusion power of a single locus is rather limited even at most polymorphic loci, but their cumulative values rise to 99% and over when just five or six most polymorphic microsatelites were analysed. The parentage indices calculated for Greyhounds, Labradors anf German shepherds allowed us to successfully resolve the paternity or parentage disputes within these breeds even without the samples from all potential parents. These population data and rather similar population structures of most contemporary dog breeds allowed us to assume that approximately ten highly polymorphic loci should suffice to reach the requested exclusion power in any pedigree breed. When this assumption is confirmed, the utility of presented data can be extended to other breeds without additional labour or financial burden.
Descriptors		PATERNITY POLYMORPHISM (GENETICS) MICROSATELLITE REPEATS DOGS POLYMERASE CHAIN REACTION