Author/Editor		Maček-Lebar, Alenka; Serša, Gregor; Čemažar, Maja; Miklavčič, Damijan
Title		Elektroporacija
Translated title		Electroporation
Type		članek
Source		Med Razgl
Vol. and No.		Letnik 37, št. 3
Publication year		1998
Volume		str. 339-54
Language		slo
Abstract		Electroporation is a process, which causes transient high-permeability of the cell membrane due to application of electric field. In the state of high-permeability, membrane allows different kinds of small and large molecules to be introduced into the cytoplasm, although the cell plasma membrane represents a formidable barrier for them in its normal state. Electroporation is governed by many parameters. Parameters of applied electric field which affect electroporation are the electric field strength, number of pulses, duration of each pulse and applied frequency. Electroporation is also affected by other parameters such as temperature, conductivity of the poration medium, osmotic pressure and cytoskeleton integrity. Transient high-permeability state induced by applied electric field can be obtained on each and every cell, it does not affect cell viability, it does not affect cell organels and causes minimal irregularities in membrane functions. Therefore, applicability of electroporation is wide. It is a phenomenon which is already widely used in biotechnology cell biology and its importance in clinical practice is growing.
Summary		Elektroporacija je pojav, pri katerem se zaradi prisotnosti električnega polja začasno poveča prepustnost celične membrane. Ob povečani prepustnosti lahko v celico vstopijo različne majhne in velike molekule, za katere je sicer celična membrana nepremagljiva ovira. Na elektroporacijo vplivajo številni dejavniki. Kako bo elektroporacija potekala, je odvisno od parametrov, ki določajo električno polje: električne poljske jakosti, trajanja posameznega pulza, števila in frekvence pulzov, pa tudi od drugih fizikalnih dejavnikov, na primer temperature, prevodnosti poracijskega medija, ozmotskega tlaka in popolnosti celičnega skeleta. Z električnim poljem lahko povečamo prepustnost celične membrane vsaki celici. Električno polje nizkih jakosti ne prizadene celičnega preživetja ali celičnih organelov in povzroči le minimalne motnje membranskih funkcij. Te lastnosti so vzrok njene široke uporabnosti. Elektroporacija je pojav, ki ga s pridom uporabljajo v biokemičnih in celičnih laboratorijih, čedalje večje pa so tudi njene možnosti za uporabo pri kliničnem delu.
Descriptors		ELECTROPORATION CELL MEMBRANE PERMEABILITY DRUG THERAPY GENE THERAPY ANTIBIOTICS ADMINISTRATION, CUTANEOUS