Author/Editor		Strojan, P; Budihna, M; Šmid, L; Vrhovec, I; Škrk, J
Title		Urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor type 1 (PAI-1) in tissue and serum of head and neck squamous cell carcinoma patients
Type		članek
Source		Eur J Cancer
Vol. and No.		Letnik 34, št. 8
Publication year		1998
Volume		str. 1193-7
Language		eng
Abstract		The aim of this study was to determine urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor type 1 (PAI-1) concentrations in tumor and adjacent normal tissue samples from 58 patients, and in serum samples from 40 of 58 patients with squamous cell carcinoma of the head and neck obtained at diagnosis and after competition of therapy. uPA and PAI/1 serum concentrations were also measured in 28 healthy volunteers who served as controls. Measurements were made using enzyme-linked immunosorbent assay (ELISA) tehniques. For both uPA and PAI-1, significantly elevated concentrations were measured in tumor tissue as compared with normal tissue (uPA: 8.89 versus 0.41 ng/mg total protein (mgp), P<0.0001; PAI-1: 23.9 versus 1.47 ng/mgp, P,0.0001). A statistically significant difference in uPA concentrations was found between normal laryngeal and nonlaryngeal tissue (0.52 versus 0.3 ng/mgp, P=0/008), and in PAI-1 concentrations between T1+2 and T3+4 stage of disease (17.32 versus 35.63 ng/mgp, P=0.04). The uPA concentrations positively correlated with those of PAI-1 measured in both tumor (RS=0.62, P<0.0001) and normal tissue (RS=0.03, P=0.02). In serum samples, lower concentrations of PAI/1 were measured in the control group that in patients with cancer (412.0 versus 680.5 ng/ml serum (mls), P=0.0006). The time of collection of the serum sample did not influence uPA and PAI-1 concentrations, and no association was observed between their concentrations and any clinical and histopatological prognostic factors tested. Our results indicate that both uPA and PAI-1 may play a specific role in process of invasion and metastasis, and might also be of prognostic value in squamous cell carcinoma of the head and neck.
Descriptors		HEAD AND NECK NEOPLASMS UROKINASE PLASMINOGEN ACTIVATOR INHIBITOR 1 CARCINOMA, SQUAMOUS CELL NEOPLASM STAGING