| Author/Editor | Čemažar, Maja | |
| Title | Protitumorsko delovanje elektrokemoterapije s cisplatinom na eksperimentalnih tumorskih modelih in vitro in in vivo | |
| Translated title | Antitumor effectiveness of Elctrochemotherapy with cisplatin on experimental tumor models in vitro and in vivo | |
| Type | monografija | |
| Place | Ljubljana | |
| Publisher | Medicinska fakulteta | |
| Publication year | 1998 | |
| Volume | str. 170 | |
| Language | slo | |
| Abstract | Electropermeabilization is a process, which causes transient high-permeability of the plasma membrane due to application of high intense electric pulses.The therapy that combines the use of electric pulses and chemotherapy is named elecrochemotherapy. The applicaton of electric pulses to tumors enables acces of bigger amount of chemotherapeutic agent into cells and hence improves antitumor effectiveness. In order to be effective in combination with electric pulses, the drug must have intracellurar site of action and hampered transport through plasma membrane. Bleomicyn meets all these requirements. Its antitumor effectiveness is highly potentianed when combined with electric pulses. Cisplatin, a chemotherapeutic drug widely used in clinical chemotherapy, is also appropriate for the use in chemotherapy. The purpose of the thesis was to test whether the cisplatin antitumor effectiveness is increased in combination with electic pulses. Antitumor effectiveness of electrochemotherapy with cisplatin was evaluated with different approaches on mouse and human tumor cell lines in vitro and in vivo. First, we determined the most appropriate electric field intensity for effective electropermeabilization in vitro, which was important for further studies on electrochemotherapy. Our results showed that electropermeabilzation of almost all tested cell lines occured at 600V/cm. On the contrary, a marked difference in electrosensitivity existed among these cell lines determined by clonogenic or MTT assay. Electochemotherapy in vitro was tested on B16 melanima, IGROV 1 cells sensitive to cisplatin and IGROV1/CDDP resistant to cisplatin. Cisplatin cytotoxicity was potentiated up to 70-times when the cells were exposed to electric pulses. ( Abstract truncated at 2000 characters) | |
| Descriptors | CELL TRANSFORMATION, NEOPLASTIC NEOPLASMS, EXPERIMENTAL CISPLATIN ELECTRIC STIMULATION THERAPY MICE TREATMENT OUTCOME |