Author/Editor		Strojnik, Tadej
Title		Imunolokaliziranje proteoliznega encima katepsina B pri človeških gliomih in ugotavljanje njegove morebitne prognostične vrednosti
Type		monografija
Place		Ljubljana
Publisher		Medicinska fakulteta
Publication year		1998
Volume		str. 83
Language		slo
Abstract		Backgrounds: Glioma is the most common form of brain tumors. As tumor cells spread early from the primary site into the surrounding brain, one of the key feature of glioma is its local invasive growth. Therefore, the major clinical problem in treatment of primary brain tumors is the recurrence even after apparently radical removal. Presumably, the invasion process is associated with altered activity and regulation of proteases and their endogenous inhibitors. The role of proteases in brain tumor invasion has not been investigated to a great extent. The aim of our study was to investigate the presence and distribution of lysosomal cystein protease, cathepsin B and its intracellular inhibitor, stefin A, in human brain tumors by immunohistochemical methods. Secondly, we investigated a correlation of cathepsin B with clinical and histopathological characteristics of the progression of the disease and possible impact of this cysteine protease on disease outcome. Patients and Methods: Hundred patients with primary neuroepithelial tumors, treated in the Maribor Teaching Hospital between 1986 and 1996, were included in the study. The stored histological slides were reviewed. Representative sections were treated with antibodies against cathepsin B and stefin A and analysed. We evaluated the frequency and the intensity of staining in tumor cells, endothelial cells and macrophages. The statistical analysis comprised descriptive methods and survival analysis. Results: The patients suffer from 27 benign tumors and 73 maligant tumors, including 58 glioblastoma multiforme. Stefin A immunostained only in a few cases, while cathepsin B stained positive in all cases. Overall immunohistochemical score (in tumor and endothelial cells) in benign compared to malignant tumors was significantly different (<*0.001, i.e. p=0.00003). (Abstract truncated at 2000 characters).
Descriptors		GLIOMA CATHEPSIN B CYSTEINE PROTEINASE INHIBITORS BRAIN NEOPLASMS PROGNOSIS IMMUNOHISTOCHEMISTRY SURVIVAL ANALYSIS NEOPLASM INVASIVENESS TREATMENT OUTCOME