Author/Editor		Vitas, Marko; Smith, Kelvin E; Plavec, Janez; Kesselmeier, Juergen; Pajič, Tadej; Ferlan, Andrej; Žigon, Dušan; Kelly, Steven L; Komel, Radovan
Title		Induction of steroidal hydroxylase activity by plant defence compounds in the filamentous fungus Cochliobolus lunatus
Type		članek
Source		Chemosphere
Vol. and No.		Letnik 38, št. 4
Publication year		1999
Volume		str. 853-63
Language		eng
Abstract		We investigated the hypothesis that the endogenous role of the commercially important inducible steroid hydroxylase cytochrome P450s of fungi was in defense against plant toxophores/secondary metabolites. Two plant defense compounds, the aglycones tomatidine and solanidine, the steroidal glycoalkoloid alpha-tomatine and the triterpene saponin beta-escin were tested as inducers of 11 beta/14 alpha-steroid hydroxylase in the filamentous fungus Conchliobolus lanatus. The extracts of saponins from the roots of Primula veris and green oat leaves were also tested as inducers of 11 beta/14 alpha-hydroxylation ectivity in progesterone biotransformation with the same fungus. induction of steroid hydroxylase and inhibition of activity in some cases support our hypothesis that their endogenous function is in biochemical defence against secondary metabolites. 4-Pregnene-3, 11, 20-trione was added as a substrate for biotransformation with. C. lunatus. We isolated from culture broth 14 alpha-hydroxy-4-pregnene-3, 11, 20-trione, and the hitherto unreported compounds, 7 alpha, 14 alpha-hydroxy-pregnene-3, 11, 20-trione and 7 alpha-hydroxy-pregna-4,8(14)-diene-3, 11, 20-trione.
Descriptors		ASCOMYCETES PROGESTERONE BIOTRANSFORMATION PREGNENES SOLANINE TOMATINE STEROID HYDROXYLASES CYTOCHROME P-450 CHROMATOGRAPHY, HIGH PRESSURE LIQUID SPECTRUM ANALYSIS, MASS NUCLEAR MAGNETIC RESONANCE