Author/Editor     Vitas, Marko; Smith, Kelvin E; Plavec, Janez; Kesselmeier, Juergen; Pajič, Tadej; Ferlan, Andrej; Žigon, Dušan; Kelly, Steven L; Komel, Radovan
Title     Induction of steroidal hydroxylase activity by plant defence compounds in the filamentous fungus Cochliobolus lunatus
Type     članek
Source     Chemosphere
Vol. and No.     Letnik 38, št. 4
Publication year     1999
Volume     str. 853-63
ISSN     0045-6535
Language     eng
Abstract     We investigated the hypothesis that the endogenous role of the commercially important inducible steroid hydroxylase cytochrome P450s of fungi was in defense against plant toxophores/secondary metabolites. Two plant defense compounds, the aglycones tomatidine and solanidine, the steroidal glycoalkoloid alpha-tomatine and the triterpene saponin beta-escin were tested as inducers of 11 beta/14 alpha-steroid hydroxylase in the filamentous fungus Conchliobolus lanatus. The extracts of saponins from the roots of Primula veris and green oat leaves were also tested as inducers of 11 beta/14 alpha-hydroxylation ectivity in progesterone biotransformation with the same fungus. induction of steroid hydroxylase and inhibition of activity in some cases support our hypothesis that their endogenous function is in biochemical defence against secondary metabolites. 4-Pregnene-3, 11, 20-trione was added as a substrate for biotransformation with. C. lunatus. We isolated from culture broth 14 alpha-hydroxy-4-pregnene-3, 11, 20-trione, and the hitherto unreported compounds, 7 alpha, 14 alpha-hydroxy-pregnene-3, 11, 20-trione and 7 alpha-hydroxy-pregna-4,8(14)-diene-3, 11, 20-trione.
Descriptors     ASCOMYCETES
PROGESTERONE
BIOTRANSFORMATION
PREGNENES
SOLANINE
TOMATINE
STEROID HYDROXYLASES
CYTOCHROME P-450
CHROMATOGRAPHY, HIGH PRESSURE LIQUID
SPECTRUM ANALYSIS, MASS
NUCLEAR MAGNETIC RESONANCE