Author/Editor		Poljak, Mario; Brenčič, Anja; Seme, Katja; Vince, Adriana; Marin, Irena J
Title		Comparative evaluation of first and second-generation Digene hybrid capture assay for detection of human papillomaviruses associated with high or intermediate risk for cervical cancer
Type		članek
Source		J Clin Microbiol
Vol. and No.		Letnik 37, št. 3
Publication year		1999
Volume		str. 796-7
Language		eng
Abstract		In the present study we comparatively evaluated the first- and second-generation Digene Hybrid Capture assays for detection of human papillomaviruses (HPV) associated with high or intermediate risk for cervical cancer in cervical specimens. Concordant results were obtained with 46A of 483 (96.8%) specimens. All 15 specimens which gave repeatedly discordant results were positive by the second-generation test onlv, and 14 of them tested PCR positive. The enhanced sensitivity of the second-generation assay is mainly a result of the reformulation of hybridization reagents and, to a lesser extent, a result of the addition of new HPV probes.
Descriptors		PAPILLOMAVIRUS, HUMAN CERVIX NEOPLASMS RISK FACTORS POLYMERASE CHAIN REACTION VAGINAL SMEARS NUCLEIC ACID HYBRIDIZATION DNA, VIRAL